Scopus
YÖKSİS Eşleşti
Proteolysis pattern and functional peptides in artisanal Tulum cheeses produced from Mut province in Turkey
LWT · Eylül 2021
YÖKSİS Kayıtları
Proteolysis pattern and functional peptides in artisanal Tulum cheeses produced from Mut province in Turkey
LWT-Food Science and Technology · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ ÇİĞDEM KONAK GÖKTEPE →
Proteolysis pattern and functional peptides in artisanal Tulum cheeses produced from Mut province in Turkey
LWT-Food Science and Technology · 2021 SCI-Expanded
PROFESÖR NİHAT AKIN →
Makale Bilgileri
DergiLWT
Yayın TarihiEylül 2021
Cilt / Sayfa149
Scopus ID2-s2.0-85107943398
Özet
The main purpose of this study was to determine proteolysis model and peptide diverseness of goat skin Tulum cheese using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses, respectively. Furthermore, bioactive properties of identified peptide sequences were investigated using online databases. Tulum cheeses produced from raw milk (without starter culture) by traditional methods in Mut province (Turkey) were obtained from local producers following their production and analyzed during 180 days of ripening. The evaluation of electrophoretic profiles by SDS-PAGE analysis indicated that 51.96% of αs-casein and 56.08% of β-casein hydrolyzed at the end of the cheese ripening. According to mass spectrometry results, 179 peptides were sequenced; of these, 65 from αs1-casein, 7 from αs2-casein, and 107 from β-casein. However, κ-casein derived peptides were not determined in Tulum cheeses during ripening. Generally, αs1-casein derived peptides originated from the N-terminal part of αs1-casein while β-casein derived peptides arose from the C-terminal part of precursor protein. Ten different functional activities ACE inhibitors, antimicrobial, antioxidant, anticarcinogenic, GLP-1 enhancer, DPP-4 inhibitor, immunomodulator, brain function enhancer, antidiabetic, and cathepsin B inhibitor have been detected. Among these functional features, ACE inhibitory peptides were found to have higher counts.
Yazarlar (3)
1
Hale İnci Öztürk
ORCID: 0000-0001-8334-0403
2
Çiğdem Konak Göktepe
3
Nihat Akın
ORCID: 0000-0002-0966-1126
Anahtar Kelimeler
Bioactive peptides
Peptide dynamics
Proteolysis
Ripened cheese
Tulum cheese
Kurumlar
Konya Gida ve Tarim Üniversitesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
3
Yazar
5
Anahtar Kelime