Scopus
YÖKSİS Eşleşti
In vitro assessment of probiotic and virulence potential of Enterococcus faecium strains derived from artisanal goatskin casing Tulum cheeses produced in central Taurus Mountains of Turkey
LWT · Nisan 2021
YÖKSİS Kayıtları
In vitro assessment of probiotic and virulence potential of Enterococcus faecium strains derived from artisanal goatskin casing Tulum cheeses produced in central Taurus Mountains of Turkey
LWT · 2021 SCI-Expanded
DOÇENT TALHA DEMİRCİ →
In vitro assessment of probiotic and virulence potential of Enterococcus faecium strains derived from artisanal goatskin casing Tulum cheeses produced in central Taurus Mountains of Turkey
LWT · 2021 SCI-Expanded
PROFESÖR NİHAT AKIN →
Makale Bilgileri
DergiLWT
Yayın TarihiNisan 2021
Cilt / Sayfa141
Scopus ID2-s2.0-85100164383
Özet
In the present study, nine Enterococcus (Ent.) faecium isolates were isolated from Tulum cheese produced traditionally in the central Taurus Mountains of Turkey during ripening period and were characterized to investigate their probiotic potential via gastrointestinal resistance, and adhesion ability, functional properties tests, and safety (haemolytic activity, antibiotic resistance, biogenic amine production, and PCR detection of some virulence factors) analyses. All of these strains proved to be resistant to bile salt and simulated gastric juice. All the isolates displayed good safety assessment regarding vancomycin susceptibility except for isolate 1050. Additionally, the majority of isolates showed high exopolysaccharide production while isolate 12 showed a desirable cholesterol removal rate. Also, five of Ent. faecium isolates indicated antibacterial activity against both Listeria monocytogenes ATCC 7644 and Staphylococcus aureus ATCC 25923. Isolates 394 and 894 were examined based on the presence of virulence genes encoding for aggregation substance (asa1), cytolysin (CylLL, CylLs, CylM, CylB, CylA), gelatinase (gelE), and hyaluronidase (hylefm). Isolate 894 harbored three virulence determinants including asa1, gelE and CylA while the virulence cylLL,S, cylM, Hylefm, gelE, and CylA genes were detected in isolate 394. Overall, isolate 894 was shown to be a potential candidate for further in vivo investigations as a probiotic culture.
Yazarlar (3)
1
Edibe Rabia Özkan
2
Talha Demirci
3
Nihat Akın
ORCID: 0000-0002-0966-1126
Anahtar Kelimeler
Ent. faecium
Probiotic
Tulum cheese
Virulence
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
32
Atıf
3
Yazar
4
Anahtar Kelime