Background: Biological control agents are increasingly used as alternatives to chemical methods against plant diseases. This study aimed to identify native bacterial isolates from plant and soil samples capable of suppressing Rhizoctonia solani induced root rot in bean (Phaseolus vulgaris L.). Given the importance of dry bean production in Turkey, especially in Konya, a key center in Central Anatolia, isolates from this region were prioritized. Methods: A total of 175 bacterial isolates were collected from rhizosphere and various tissues of beans and Datura spp. Their antagonistic activity against R. solani AG-4 was assessed via in vitro dual culture assays and in vivo experiments under controlled conditions using a Randomized Complete Block Design (RCBD). Identification of effective isolates was performed using 16S rRNA gene sequencing. Result: Ten isolates strongly inhibited fungal growth in vitro, with endophytes Bacillus pumilus DP25 and B. velezensis DP98 showing complete (100%) suppression. In vivo, B. pumilus DP25 (87%), B. halotolerans DP48 (82%) and B. amyloliquefaciens DP76 (80%) significantly reduced disease severity; other isolates showed 53-78% efficacy. The study highlights the potential of native Bacillus spp. as biocontrol agents, offering practical insights for sustainable bean cultivation in Turkey.