This study evaluated the effects of extracts prepared using two different methods (decoction extraction and soxhlet ethyl acetate/hexane extraction) from Inula viscosa on H2O2-induced oxidative stress and wound healing model in mouse L929 fibroblast cells. The cytotoxic effect started to disappear statistically (p<0.05) at concentrations of Soxhlet ethyl acetate/hexane extracts (SoxEHE) in and below 0.1 mg/ mL, while the same effect was observed at concentrations of decoction extracts (DE) in and below 0.2 mg/mL. Therefore, 0.2 and 0.02 mg/ mL concentrations of DE, and 0.1 and 0.01 mg/mL concentrations of SoxEHEs were used. While cell migration was positively affected in all research concentrations, statistically significant results (p<0.05) were obtained from 0.2 mg/mL of DE and 0.1 mg/mL of SoxEHE extracts. Malondialdehyde (MDA) levels were found to be statistically (p <0.05) decreased, but COL1A1 levels were higher in cell lines treated with oxidative stress + extract than in the cell line treated only with H2O2, and reduced glutathione (GSH) levels were higher in cell lines only treated only with extract than in oxidative stress-induced cell lines. Consequently, it has been observed that the extracts have positive effects on migration and oxidative stress. Therefore, I. viscosa may serve as a new therapeutic agent for wound healing.