Objective: This study aimed to analyze the chemical and functional properties of fruits from five selected Prunus spinosa L. genotypes grown in Seydişehir district of Konya province, and determine the differences among the genotypes. Material and Methods: In the research, fruit properties such as fruit weight, pericarp color, flesh color were determined, while total soluble solids (TSS), pH, titratable acid (TA), total phenol content, antioxidant level and volatile organic compounds were analyzed. PCA analysis was employed to distinguish variations among the genotypes. Results: Five different blackthorn genotypes were examined for fruit properties, revealing variations. Fruit weight ranged from 1.31 g to 2.67 g. TSS content was determined to be between 11.9% and 13.2%. pH values varied from 3.35 to 4.22, while TA ranged from 0.83% to 1.30%. Total phenol content ranged from 356.92 mg GAE/100 g to 387.56 mg GAE/100 g. Total antioxidant levels were determined to be between 65.13% and 77.06%. Thirty different compounds were detected in the analysis of volatile organic compounds, including seven different acids. Conclusion: Significant diversity among the blackthorn genotypes has been identified. Statistical analyses have shown that B01T genotype has the highest total phenol content, while B05T genotype has the highest antioxidant level. The presence of various volatile organic compounds in blackthorn indicates the diversity of its chemical profile. The findings support the notion that blackthorn has a beneficial potential for health and is a valuable candidate for the development of health-focused food products.