In this study, the effects of different NaCl levels (0, 50, 100, 150 mM) on ‘Viking’ aronia variety plants in the in vitro culture medium were investigated. In the study, aronia plantlets were cultured in the MS and the ½MS rooting media with 1 mg l−1 IBA supplemented with different concentrations of NaCl for 35 days. At the end of salinity stress treatments, plant height, leaf area, plant fresh and dry weight, rooting rate, root number, root length as well as root fresh and dry weight were measured. In all measurements recorded for salinity stress, there was a decrease in all growth parameters as the NaCl level increased in both media. The ½MS medium compared to the full MS medium performed better at almost all growth parameters at every NaCl concentration. The average highest plant height was recorded in the control treatment (4.77 cm) of the ½MS medium; the lowest plant height was recorded in 150 mM NaCl treatment (1.67 cm) of the MS medium. The lowest leaf area was determined at 150 mM NaCl concentration, and it reduced 68.8% in the MS medium and 73% in the ½MS medium. Rooting was highest in the control groups (100%) in both media; a significant gradual decrease occurred in 50 and 100 mM NaCl concentration, and rooting did not occur in 150 mM NaCl treatment. In addition, with increasing salt concentration in the culture medium, both root length and number decreased. Consequently, it has been determined that the ‘Viking’ aronia variety is sensitive to high salinity levels.