The aerial part, root and fruits of Ferulago longistylis Boiss. an endemic species, were subjected to the evaluation of essential oil compositions and antimicrobial activity. The essential oils were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The main components of the aerial part were identified as α-pinene (18.7%), bornyl acetate (11.8%), 2,3,6trimethyl benzaldehyde (9.3%), p-cymene (7.7%), for the roots, α-pinene (91.7%); and for the fruits, 2,3,6-trimethyl benzaldehyde (26.5%), α-pinene (14.9%), (Z)-β-ocimene (14.1%), myrcene (7.5%), sabinene (7.3%), respectively. The essential oils were evaluated for their in vitro antimicrobial activity against a panel of some human pathogens using a broth microdilution technique resulting in relatively weak antimicrobial and antifungal activity (MIC 1.25-10 mg/mL).