Scopus Eşleşmesi Bulundu
1
Atıf
45
Cilt
212-220
Sayfa
🔓
Açık Erişim
Scopus Yazarları: Mustafa Emin Oz, Emrah Torlak, Oğuzhan Avci, Müge Dogan, Ali Tevfik Uncu
Özet
The present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit of detection obtained was approximately 12 RNA copies per reaction corresponding to a CT value of 36.79. According to the results, it was observed that the developed in-house method has a specific, sensitive, and powerful detection limit as much as the gold standard test. As a result, it was concluded that the proposed method, which was developed as a more costeffective and easily optimized analysis, could provide an advantage for detecting SRMV in diagnostic laboratories.
Anahtar Kelimeler (Scopus)
Real-time RT-PCR
SRMV
Evagreen
Fusion glycoproteingene
Low-cost diagnosis
Scimago Dergi Bilgisi
Otomatik ISSN Eşleştirmesi
2021 yılı verileri
Turkish Journal of Veterinary and Animal Sciences
Q3
SJR Quartile
0,209
SJR Skoru
44
H-Index
🔓
Açık Erişim
Kategoriler: Veterinary (miscellaneous) (Q3)
Alanlar: Veterinary
Ülke: Turkey
· TUBITAK
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Anahtar Kelimeler
Real-time RT-PCR
SRMV
Evagreen
Fusion glycoproteingene
Low-cost diagnosis
Makale Bilgileri
Dergi
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
ISSN
1300-0128
Yıl
2021
/ 1. ay
Makale Türü
Özgün Makale
Hakemlik
Hakemli
Endeks
C.A.B. International
TEŞV Puanı
12,00
Yayın Dili
Türkçe
Kapsam
Uluslararası
Toplam Yazar
5 kişi
Erişim Türü
Elektronik
Erişim Linki
Makaleye Git
Alan
Sağlık Bilimleri Temel Alanı
Veterinerlik Virolojisi
YÖKSİS Yazar Kaydı
Yazar Adı
ÖZ Mustafa Emin, TORLAK Emrah, AVCI OĞUZHAN, DOĞAN MÜGE, UNCU ALİ TEVFİK
YÖKSİS ID
6052933
Hızlı Erişim
Metrikler
Scopus Atıf
1
TEŞV Puanı
12,00
Yazar Sayısı
5