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C.A.B. International Özgün Makale Scopus
A new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assay
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 2021
Scopus Eşleşmesi Bulundu
1
Atıf
45
Cilt
212-220
Sayfa
🔓
Açık Erişim
Scopus Yazarları: Mustafa Emin Oz, Emrah Torlak, Oğuzhan Avci, Müge Dogan, Ali Tevfik Uncu
Özet
The present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit of detection obtained was approximately 12 RNA copies per reaction corresponding to a CT value of 36.79. According to the results, it was observed that the developed in-house method has a specific, sensitive, and powerful detection limit as much as the gold standard test. As a result, it was concluded that the proposed method, which was developed as a more costeffective and easily optimized analysis, could provide an advantage for detecting SRMV in diagnostic laboratories.
Anahtar Kelimeler (Scopus)
Real-time RT-PCR SRMV Evagreen Fusion glycoproteingene Low-cost diagnosis
Scimago Dergi Bilgisi Otomatik ISSN Eşleştirmesi 2021 yılı verileri
Turkish Journal of Veterinary and Animal Sciences
Q3
SJR Quartile
0,209
SJR Skoru
44
H-Index
🔓
Açık Erişim
Kategoriler: Veterinary (miscellaneous) (Q3)
Alanlar: Veterinary
Ülke: Turkey · TUBITAK
Bu bilgiler makale yılına göre Scimago veritabanından ISSN eşleştirmesiyle otomatik getirilmektedir. Dergi sıralama verileri Scimago'nun ilgili yılı baz alınmaktadır.

Anahtar Kelimeler

Real-time RT-PCR SRMV Evagreen Fusion glycoproteingene Low-cost diagnosis

Makale Bilgileri

Dergi TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
ISSN 1300-0128
Yıl 2021 / 1. ay
Makale Türü Özgün Makale
Hakemlik Hakemli
Endeks C.A.B. International
TEŞV Puanı 12,00
Yayın Dili Türkçe
Kapsam Uluslararası
Toplam Yazar 5 kişi
Erişim Türü Elektronik
Erişim Linki Makaleye Git
Alan Sağlık Bilimleri Temel Alanı Veterinerlik Virolojisi

YÖKSİS Yazar Kaydı

Yazar Adı ÖZ Mustafa Emin, TORLAK Emrah, AVCI OĞUZHAN, DOĞAN MÜGE, UNCU ALİ TEVFİK
YÖKSİS ID 6052933

Metrikler

Scopus Atıf 1
TEŞV Puanı 12,00
Yazar Sayısı 5