Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q3
A new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assay
Turkish Journal of Veterinary and Animal Sciences · Ocak 2021
YÖKSİS Kayıtları
A new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assay
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES · 2021 C.A.B. International
Prof. Dr. OĞUZHAN AVCI →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Three dimensional reconstruction of multidetector computed tomography images of paranasal sinuses of New Zealand rabbits
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2000 ISSN: 1300-0128 SCI
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Effect of low birthweight on serum thyroid hormones glucose urea and blood pH in newborn lambs
2003 ISSN: 1300-0128 SCI 11 atıf
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1997 ISSN: 1300-0128 SCI 6 atıf
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1900 ISSN: 1300-0128 SCI-Expanded 13 atıf
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1999 ISSN: 1300-0128 SCI-Expanded 45 atıf
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2006 ISSN: 1300-0128 SCI-Expanded 2 atıf
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2002 ISSN: 1300-0128 SCI-Expanded 2 atıf
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2001 ISSN: 1300-0128 SCI-Expanded 5 atıf
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2000 ISSN: 1300-0128 SCI-Expanded 3 atıf
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2000 ISSN: 1300-0128 SCI-Expanded 4 atıf
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Efficacy of experimental inactivated and live Rhodococcus equivaccines for thoroughbred Arabian mares in mice
2015 ISSN: 13000128 SSCI
Prof. Dr. OSMAN ERGANİŞ →
Immunohistopathological and biochemical study of the effects of dead nettle Urtica dioica extract on preventing liver lesions induced by experimental aflatoxicosis in rats
2015 ISSN: 1300-0128 SCI-Expanded
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Canine brucellosis in Turkey from an environmental perspective
2016 ISSN: 1300-0128 SCI-Expanded
Prof. Dr. UÇKUN SAİT UÇAN →
Mycoplasma infections in dairy cattle farms in Turkey
2016 ISSN: 13000128 SCI-Expanded
Prof. Dr. ZAFER SAYIN →
Conservation characteristics and protein fractions of cereal silages ensiled with additives at the booting and dough stages of maturity
2014 ISSN: 13000128 SCI-Expanded
Prof. Dr. MUSTAFA SELÇUK ALATAŞ →
Gonadotrophin Concentrations in Sheep Plasma after Injections ofGnRH with or without a Mixture of Serine and Threonine
2005 ISSN: 1300-0128 SCI-Expanded Q4
Öğr. Gör. HÜSEYİN BAKİ ÇİFTCİ →
Makale Bilgileri
ISSN13000128
Yayın TarihiOcak 2021
Cilt / Sayfa45 · 212-220
Scopus ID2-s2.0-85106226995
Erişim🔓 Açık Erişim
Özet
The present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit of detection obtained was approximately 12 RNA copies per reaction corresponding to a CT value of 36.79. According to the results, it was observed that the developed in-house method has a specific, sensitive, and powerful detection limit as much as the gold standard test. As a result, it was concluded that the proposed method, which was developed as a more costeffective and easily optimized analysis, could provide an advantage for detecting SRMV in diagnostic laboratories.
Yazarlar (5)
1
Mustafa Emin Oz
2
Emrah Torlak
3
Oğuzhan Avci
ORCID: 0000-0001-9299-4695
4
Müge Dogan
5
Ali Tevfik Uncu
Anahtar Kelimeler
Evagreen
Fusion glycoproteingene
Low-cost diagnosis
Real-time RT-PCR
SRMV
Kurumlar
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Veterinary Control and Research Institute
Konya Turkey
Scimago Dergi (ISSN Eşleşmesi)
Turkish Journal of Veterinary and Animal Sciences
Q3
OA
SJR Skoru0,256
H-Index46
YayıncıTUBITAK
ÜlkeTurkey
Veterinary (miscellaneous) (Q3)
Metrikler
2
Atıf
5
Yazar
5
Anahtar Kelime