The objective of the present investigation was to develop novel three-dimensional (3D) and biodegradable tissue scaffolds (TSs), consisting of biological polymers and simvastatin (SV)-loaded nanostructured lipid carriers (NLCs) for the treatment of diabetic wounds. NLCs were prepared via high-shear homogenization and freeze-drying was used for NLC-incorporated TSs. The resulting NLC suspension contained spherical nanosized (110–158 nm) homogeneous particles (PDI<0.16) with >99% encapsulation efficiency. The NLCs were characterized via differential scanning calorimetry and Fourier transform infrared spectroscopy analysis. TSs have 3D high porous structure (74.38%) and sufficient large pore size (∼100–200 μm), low weight loss (57.7%), high absorption capacity (710.88%) and good mechanical strength. Incorporation of NLCs into TSs delayed the initial SV release and generated a more controlled release profile. Biocompatibility of the TSs was shown using MTT assay. The results suggest that TSs can be considered as suitable candidates for diabetic wound treatment.