Many important local grape cultivars have been producing for their local preference and well adaptation in own zones. ‘Eksi Kara’ and ‘Gök Üzüm’ (Vitis vinifera L.) are ancient cultivars in the Anatolian peninsula, especially in the middle Taurus viticultural areas, and are utilized as table grape, raisin and grape juice production. Boron (B) plays a key role in fruit set and effects the formation of pollen grains and pollen tube growth, while zinc (Zn) plays an important role in different enzyme activities and cell division. Zn and B deficiencies cause significant yield losses in vineyards. ‘Eksi Kara’ has functional female flowers and it has been pollinated with hermaphroditic flowers of grape ‘Gök Üzüm’. In this study, two weeks after the fruit set, 1.5 g L-1 B (H3BO3) and 2.5 g L-1 Zn (ZnSO4) were applied separately in the 20 years old producer vineyards at 1000 m. Both cultivars were grafted on rootstocks 110R. The effects of the B and Zn treatments were evaluated by measuring berry and cluster size and fruit quality at harvest. The foliar treatments affected berry and cluster sizes and measured fruit quality values in both cultivars. Berry weight was increased 21.50% in ‘Eksi Kara’, and the cluster weight was increased 33% in ‘Eksi Kara’ by B. Berry weight was increased 18.13% in ‘Gök Üzüm’ and 9% in ‘Eksi Kara’ by Zn. The increase in cluster weight was measured 47.92 and 46.74% in ‘Eksi Kara’ and ‘Gök Üzüm’ respectively by Zn. Fruit juice yield was increased 16.18% by Zn on the other hand 22% decrease detected in ‘Gök Üzüm’ by B application. Fruit ripening process was not significantly altered by Zn while B treatment caused acceleration on fruit ripening in both cultivars. The present study reveals that effects of B and Zn were different among cultivars.