Objective: In order to detect the damage caused by obesity and hyperlipidemia to the cardiovascular system in the early period, parameters that will be used for follow up of these diseases and provide information about the changes are needed. It is of course valuable to measure multiple parameters simultaneously with fast, high efficiency and less sample quantity in the measurement of these parameters. Material and Methods: We have studied serum levels of cardiovascular risk parameters sE-Selectin, follistatin, PAPP-A, sPECAM-1, pentraxin-3 and tissue factor using the multiplex immunoassay method in women primary hyperlipidemic and obese hyperlipidemic. In addition, lipid profile, liver enzymes and blood glucose were measured by routine methods. We have studied these parameters in serum of 30 healthy women, 28 primary hyperlipidemic and 32 obese hyperlipidemic women. Results: sE-selectin (p<0.01) levels were significantly higher than control group. Serum follistatin levels were significantly higher in obese hyperlipidemic group (p<0.001) in obese hyperlipidemic group and primary hyperlipidemic group (p<0.01) compared to control group. Moreover serum sPECAM-1 (p<0.001) levels were significantly higher in obese hyperlipidemic group and primary hyperlipidemic group compared to control group. There were no significant differences between serum PAPP-A, pentraxin-3 and tissue factor levels of the groups. Conclusion:Our findings suggest that hyperlipidemia associated with obesity may be the cause of cardiovascular disease rather than hyperlipidemia.