The main objective of this study was to determine total oil, total phenol, antioxidant activity and mineral contents of hull-less pumpkin seeds and also fatty acid composition of seed oils. The results indicated that total oil, total phenol content and antioxidant activity values were found between 33.04 and 46.97 %, 56.94 and 87.15 mg GAE/100 g and 0.19 and 11.75 %, respectively (p < 0.05). Linoleic, oleic, palmitic and stearic acids were the most prominent fatty acids in all genotypes. The most abundant mineral in the studied seeds, which belong to different genotypes, was potassium (2704.75–1033.63 ppm) followed by phosphorus (3569.690–9108.835 ppm) and magnesium (1275.15–3938.16 ppm) (p < 0.05). Particularly genotype-1 was the richest seed in essential fatty acids and minerals.