Leaf analysis is used as a standard method for determination of the nutritional status of apple orchards in the middle of vegetation, but not take into consideration the differences among cultivars in the evaluation of results of leaf analysis. In 2010- 2011 years, this study was performed on leaves collected from two different regions in Isparta, Turkey where apple is cultivated for determining the effect of cultivars on evaluation of the results of leaf analysis. In the middle of vegetation, leaves were collected from 68 orchards which consist of 'Starking Delicious', 'Golden Delicious', 'Granny Smith' and 'Spur Delicious', and contents of N, P, K, Ca, Mg, Mn, Zn, and B were determined. The cultivars showed statistically significant differences in terms of N, P, K, Mg and B contents. According to the results of this study, it was determined that cultivars should be taken into consideration in the evaluation of leaf analysis.