Scopus
Can MitoTEMPO protect rat sciatic nerve against ischemia-reperfusion injury?
Naunyn Schmiedeberg S Archives of Pharmacology · Mart 2021
Makale Bilgileri
DergiNaunyn Schmiedeberg S Archives of Pharmacology
Yayın TarihiMart 2021
Cilt / Sayfa394 · 545-553
Scopus ID2-s2.0-85099098356
Özet
Abdominal ischemia-reperfusion (I/R) is known to cause both structural and functional damage to sciatic nerve which is related to the oxidative stress. We investigated the protective effects of mitochondria-targeted antioxidant (2-(2,2,6,6-tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl) triphenylphosphonium chloride (MitoTEMPO) on ischemia-reperfusion-induced nerve damage by using the conduction velocity distribution (CVD) calculations from in vitro compound nerve action potential (CNAP) recordings from rat sciatic nerve. Adult male Wistar albino rats were divided into three groups. The IR and IR + MT groups had aortic cross-clamping for 1 h followed by 2 h reperfusion, while SHAM group had the same procedure without cross-clamping. IR + MT group received 0.7 mg/kg/day MitoTEMPO injection for 28 days before I/R, while other groups received vehicle alone. Ischemia-reperfusion resulted in a significant decrease (p <.05) in maximum depolarizations (mV), areas (mV.ms), and maximum and minimum upstroke velocities (mV/ms) of CNAPs, while injection of MitoTEMPO showed a complete protective effect on these impairments. The histograms for CVD showed that I/R blocked the contribution of fast-conducting fibers (> 60 m/s). MitoTEMPO prevented that blockage and caused a shift in the CVD. Functional nerve damage caused by I/R can be prevented by MitoTEMPO, which can enter mitochondria, the main source of reactive oxygen species (ROS).
Yazarlar (4)
1
Seckin Tuncer
2
Ahmet Akkoca
ORCID: 0000-0001-8269-9385
3
Murat Cenk Celen
ORCID: 0000-0003-0727-0110
4
Nizamettin Dalkilic
Anahtar Kelimeler
Conduction velocity distribution
Ischemia-reperfusion
MitoTEMPO
Sciatic nerve
Kurumlar
Başkent Üniversitesi
Ankara Turkey
Eskişehir Osmangazi Üniversitesi Tip Fakültesi
Eskisehir Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
9
Atıf
4
Yazar
4
Anahtar Kelime