Scopus
YÖKSİS Eşleşti
Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations
International Journal of Biological Macromolecules · Eylül 2017
YÖKSİS Kayıtları
Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations
International Journal of Biological Macromolecules · 2017 SCI
PROFESÖR İDRİS SARGIN →
Makale Bilgileri
DergiInternational Journal of Biological Macromolecules
Yayın TarihiEylül 2017
Cilt / Sayfa102 · 914-923
Scopus ID2-s2.0-85018263735
Özet
Current study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA.
Yazarlar (6)
1
Muhammad Mujtaba
2
Murat Kaya
3
Lalehan Akyuz
4
Demet Erdonmez
5
Bahar Akyuz
6
Idris Sargin
ORCID: 0000-0003-3785-9575
Anahtar Kelimeler
Cu Immobilization ++
FT-IR
pUC18
SEM
TGA
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Metrikler
7
Atıf
6
Yazar
5
Anahtar Kelime