Scopus
YÖKSİS Eşleşti
The ocular endothelin system: A novel target for the treatment of endotoxin-induced uveitis with bosentan
Investigative Ophthalmology and Visual Science · Mayıs 2014
YÖKSİS Kayıtları
The Ocular Endothelin System A Novel Target for the Treatment of Endotoxin Induced Uveitis With Bosentan
Investigative Ophthalmology Visual Science · 2014 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiInvestigative Ophthalmology and Visual Science
Yayın TarihiMayıs 2014
Cilt / Sayfa55 · 3517-3524
Scopus ID2-s2.0-84902245892
Özet
Purpose. We compared the anti-inflammatory effects of bosentan and dexamethasone in endotoxin-induced uveitis (EIU). Methods. Endotoxin-induced uveitis was induced by subcutaneous injection of lipopolysaccharide (LPS, 200 μg) in Wistar rats. Rats were divided randomly into 10 groups (n = 6). Bosentan at doses of 50 and 100 mg/kg were administered orally 1 hour before and 12 hours after LPS injection, and dexamethasone was administered by intraperitoneally 30 minutes before and 30 minutes after LPS injection at a dose of 1 mg/kg. Data were collected at two time points for each control and treatment; animals were killed at either 3 or 24 hours after LPS injection. Histopathologic evaluation and aqueous humour measurements of TNF-α level were performed, and endothelin-1 (ET-1), inducible nitric oxide synthase (iNOS), and endothelin receptor A and B (EDNRA and B) expression were analyzed. Results. The group treated with 100 mg/kg bosentan at 24 hours displayed significantly milder uveitis and fewer inflammatory cells compared to LPS-injected animals, and there were similar findings in the dexamethasone-treated group at 24 hours. The TNF-α levels in the dexamethasone treatment group were lower than those in the LPS-induced uveitis control group (P < 0.05); however, there was no difference between the dexamethasone and bosentan treatment groups at 3 and 24 hours after LPS administration. Bosentan treatment at doses of 50 and 100 mg/kg significantly decreased iNOS expression compared to LPS-injected animals (P < 0.001). The ET-1 expression was suppressed significantly by bosentan and dexamethasone at 3 and 24 hours after LPS administration (P < 0.001). The EDNRA expression in the bosentan treatment groups was statistically significantly lower than that in the LPS-induced uveitis control group at 3 and 24 hours after LPS administration (P < 0.05). Conclusions. Bosentan reduces intraocular inflammation and has similar effects as dexamethasone in a rat model of EIU. © 2014 The Association for Research in Vision and Ophthalmology, Inc.
Yazarlar (10)
1
Sadullah Keles
2
Zekai Halici
3
Atmaca Hasan Tarik
4
Muhammed Yayla
ORCID: 0000-0002-0659-3084
5
Kenan Yildirim
6
Metin Ekinci
7
Erol Akpinar
8
Durdu Altuner
9
Ozgur Cakici
10
Zafer Bayraktutan
Anahtar Kelimeler
Bosentan
Endothelin-1
Endotoxin-induced uveitis
TNF-α iNOS
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Igdir State Hospital
Igdir Turkey
Kafkas Üniversitesi
Kars Turkey
Kirikkale Üniversitesi
Kirikkale Turkey
Muğla Sıtkı Koçman Üniversitesi
Mugla Turkey
Recep Tayyip Erdogan University
Rize Turkey
Metrikler
18
Atıf
10
Yazar
4
Anahtar Kelime