Scopus
YÖKSİS Eşleşti
Development and validation of UFLC-MS/MS method for determination of bosentan in rat plasma
Journal of Pharmaceutical and Biomedical Analysis · Ağustos 2014
YÖKSİS Kayıtları
Development and validation of UFLC MS MS method for determination of bosentan in rat plasma
Journal of Pharmaceutical and Biomedical Analysis · 2014 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiJournal of Pharmaceutical and Biomedical Analysis
Yayın TarihiAğustos 2014
Cilt / Sayfa97 · 33-38
Scopus ID2-s2.0-84899841439
Özet
A rapid, simple and sensitive UFLC-MS/MS method was developed and validated for the determination of bosentan in rat plasma using etodolac as an internal standard (IS) after liquid-liquid extraction with diethyl ether-chloroform (4:1, v/v). Bosentan and IS were detected using electrospray ionization in positive ion multiple reaction monitoring mode by monitoring the transitions m/. z 551.90. →. 201.90 and 288.20. →. 172.00, respectively. Chromatographic separation was performed on the inertsil ODS-4 column with a gradient mobile phase, which consisted of 0.1% acetic acid with 5. mM ammonium acetate in water for solvent A and 5. mM ammonium acetate in acetonitrile-methanol (50:50, v/v) for solvent B at a flow rate of 0.3. mL/min. The method was sensitive with 0.5. ng/mL as the lower limit of quantitation (LLOQ) and the standard calibration curve for bosentan was linear (r>. 0.997) over the studied concentration range (0.5-2000. ng/mL). The proposed method was fully validated by determining specificity, linearity, LLOQ, precision and accuracy, recovery, matrix effect and stability. The validated method was successfully applied to plasma samples obtained from rats. © 2014 Elsevier B.V.
Yazarlar (7)
1
Alptuğ Atila
2
M. Ozturk
3
Y. Kadioglu
4
Zekai Halici
5
Didar Turkan
6
Muhammed Yayla
ORCID: 0000-0002-0659-3084
7
Harun Un
ORCID: 0000-0003-1772-282X
Anahtar Kelimeler
Bosentan
Liquid-liquid extraction
Rat plasma
UFLC-MS/MS
Validation
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Ataturk University, Faculty of Medicine
Erzurum Turkey
Turkish Public Health Association
Erzurum Turkey
Metrikler
10
Atıf
7
Yazar
5
Anahtar Kelime