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What is the role of bosentan in healing of femur fractures in a rat model?

Journal of Bone and Mineral Metabolism · Eylül 2015

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YÖKSİS Kayıtları
What is the role of bosentan in healing of femur fractures in a rat model
Journal of Bone and Mineral Metabolism · 2015 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →

Makale Bilgileri

DergiJournal of Bone and Mineral Metabolism
Yayın TarihiEylül 2015
Cilt / Sayfa33 · 496-506
Özet The purpose of this study was to examine the effects bosentan (which is a strong vasoconstrictor) on bone fracture pathophysiology, and investigate the roles of the nonselective endothelin 1 receptor blocker bosentan on the bone fractures formed in rats through radiographic, histopathologic, and immunohistochemical methods. The rats were divided into three groups (six rats in each group): a femoral fracture control group, a femoral fracture plus bosentan at 50 mg/kg group, and a femoral fracture plus bosentan at 100 mg/kg group. The femoral fracture model was established by transversely cutting the femur at the midsection. After manual reduction, the fractured femur was fixed with intramedullary Kirschner wires. The radiographic healing scores of the bosentan 100 and 50 mg/kg groups were significantly better that those of the fracture control group. The fracture callus percent of new bone in the bosentan 100 mg/kg group was significantly greater than that in the control group. Also, semiquantitative analysis showed higher positive vascular endothelial growth factor and osteocalcin staining and lower positive endothelin receptor type A staining in the treatment groups than in the control group. Bosentan treatment also decreased tissue endothelin 1 expression relative to that in the fracture control group. As a result of our study, the protective effect of bosentan was shown in experimental femoral fracture healing in rats by radiographic, histopathologic, and molecular analyses.

Yazarlar (10)

1
Ali Aydin
2
Zekai Halici
3
Erol Akpinar
4
A. Murat Aksakal
5
Murat Saritemur
6
Muhammed Yayla
ORCID: 0000-0002-0659-3084
7
C. Semih Kunak
8
Elif Cadirci
9
Atmaca Hasan Tarik
10
S. Sena Karcioglu

Anahtar Kelimeler

Bone fracture Bosentan Endothelin 1 Femur Rat

Kurumlar

Atatürk Üniversitesi
Erzurum Turkey
Ataturk University, Faculty of Medicine
Erzurum Turkey
Kirikkale Üniversitesi
Kirikkale Turkey
Ordu Üniversitesi
Ordu Turkey
Sevket Yilmaz Education and Research Hospital
Bursa Turkey

Metrikler

7
Atıf
10
Yazar
5
Anahtar Kelime