Scopus
🔓 Açık Erişim
Role of endothelin 1 on proliferation and migration of human MCF-7 cells
Eurasian Journal of Medicine · Ekim 2020
Makale Bilgileri
DergiEurasian Journal of Medicine
Yayın TarihiEkim 2020
Cilt / Sayfa52 · 277-282
Scopus ID2-s2.0-85095442286
Erişim🔓 Açık Erişim
Özet
Objective: The aim of this study was to explore the role of endothelin 1 (ET-1) in human breast cancer proliferation and migration and antagonism of endothelin receptor A (ETAR) and endothelin receptor B (ETBR) by using the non-selective dual ETA/ETB receptor antagonist bosentan and determine its anti-proliferative, anti-metastatic, and apoptotic effects demonstrated by nuclear factor kappa B (NF-kB), vascular endothelial growth factor (VEGF), Caspase 3 and Caspase 9 expression on endothelin-induced proliferation of MCF-7 cell line in vitro. Materials and Methods: A total of 8,000 cells were seeded into e-plates 24 hours after the cells were incubated with or without 10-4 M BOS (1 hour before ET-1 treatment); 10-7, 10-8, and 10-9 M ET-1 for 1-4 days. Results: Whether ET-1 is present or not in the tumor area, bosentan exerts anti-proliferative effect on breast cancer. However, ET-1 and bosentan group showed important inhibitory effect on tumor migration compared to bosentan alone, which can be attributed to increased activity of ET-1 axis in the presence of ET-1. The imbalance among the NF-kB, caspases, and VEGF, which are predictive factors of carcinogenesis significantly improved after bosentan administration. Conclusion: Our study definitely demonstrated ET-1 and its critical role in cancer progression with apop-totic and anti-apoptotic pathways (NF-κB) and VEGF expression, and migration analyses were also per-formed. The second major finding was that bosentan inhibited ET-1-mediated effects on tumor proliferation and migration.
Yazarlar (5)
1
Irfan Cinar
ORCID: 0000-0002-9826-2556
2
Muhammed Yayla
ORCID: 0000-0002-0659-3084
3
Muhammet Celik
4
Arzu Bilen
5
Zafer Bayraktutan
Anahtar Kelimeler
Bosentan
Cancer
ET-1
MCF-7
Metastasis
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Kastamonu University
Kastamonu Turkey
Metrikler
4
Atıf
5
Yazar
5
Anahtar Kelime