Scopus
YÖKSİS Eşleşti
In Vivo and In Vitro Cardioprotective Effect of Gossypin Against Isoproterenol-Induced Myocardial Infarction Injury
Cardiovascular Toxicology · Ocak 2022
YÖKSİS Kayıtları
In Vivo and In Vitro Cardioprotective Effect of Gossypin Against Isoproterenol-Induced Myocardial Infarction Injury
Cardiovascular Toxicology · 2022 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiCardiovascular Toxicology
Yayın TarihiOcak 2022
Cilt / Sayfa22 · 52-62
Scopus ID2-s2.0-85116026943
Özet
The aim of the study was to examine the protective effects and possible mechanism of gossypin against isoproterenol (ISO)-mediated myocardial damage in vivo and H9c2 cell damage in vitro. H9c2 cells were categorized into five groups. Viability was evaluated with MTT and LDH release in H9c2 cells. Apoptotic parameter analysis was performed with cytochrome c (Cyt-c), caspase-3 (CASP-3), and BCL2/Bax mRNA expression levels. In vivo, gossypin was administered orally to mice at doses of 5, 10, and 20 mg/kg for 7 days. ISO groups were injected with isoproterenol (150 mg/kg) subcutaneously (on 8th and 9th) for 2 days. Afterward, lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) levels and Troponin-I (Tn-I) amount from their serum, oxidative stress parameters superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels, and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1 β), and NF-kB mRNA expression levels with inflammatory markers from heart tissue were evaluated. In addition, IL-1B, BCL-2, and cas-3 immunohistochemical staining was performed from heart tissue and TNF-a level was measured by ELISA method. Administration of Gossypin protected the cells by dose-dependent, eliminating the reduced cell viability and increased LDH release of ISO in H9c2 cells. In mice serum analyses, increased LDH, CK-MB levels, and Tn-I levels were normalized by gossypin. ISO administration in heart tissue is regulated by gossypin with increased SOD activity, GSH amount, TNF-α, IL-6, IL-1β, and NF-kB mRNA expression levels and decreased MDA amount. Overall, the present results demonstrated that gossypin has a potential cardioprotective treatment for ischemic heart disease on in vivo and in vitro.
Yazarlar (7)
1
Irfan Cinar
ORCID: 0000-0002-9826-2556
2
Muhammed Yayla
ORCID: 0000-0002-0659-3084
3
Taha Tavaci
4
Erdem Toktay
ORCID: 0000-0002-0715-2707
5
Rustem Anil Ugan
ORCID: 0000-0002-4837-2343
6
Pinar Bayram
ORCID: 0000-0001-9924-7051
7
Hamza Halici
Anahtar Kelimeler
Antioxidant
Cardioprotection
Gossypin
Isoproterenol
Myocardial infarction
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Ataturk University, Faculty of Medicine
Erzurum Turkey
Kafkas Üniversitesi
Kars Turkey
Kastamonu University
Kastamonu Turkey
Metrikler
22
Atıf
7
Yazar
5
Anahtar Kelime