Scopus
YÖKSİS Eşleşti
Protective effects of melatonin receptor agonists on endotoxin-induced uveitis in rats
Iranian Journal of Basic Medical Sciences · Mayıs 2023
YÖKSİS Kayıtları
Protective effects of melatonin receptor agonists on endotoxin-induced uveitis in rats
Iranian Journal of Basic Medical Sciences · 2023 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiIranian Journal of Basic Medical Sciences
Yayın TarihiMayıs 2023
Cilt / Sayfa26 · 540-548
Scopus ID2-s2.0-85153195136
Özet
Objective(s): Melatonin has an important role in regulating a variety of physiological functions of the body. We investigated the protective effects of Agomelatine (AGO) and Ramelteon (RAME) on Endotoxin-Induced Uveitis (EIU) in rats. Materials and Methods: 70 rats were randomly divided into fourteen groups. Healthy group normal saline, (IP), Uveitis group (200 μg/kg lipopolysaccharide (LPS), SC), DEX group (200 μg/kg LPS plus 1 mg/kg dexamethasone, IP), AGO20 group received 200 μg/kg LPS plus 20 mg/kg AGO, AGO40 group received 200 μg/kg LPS plus 40 mg/kg AGO, RAME2 group received 200 μg/kg LPS plus 2 mg/kg RAME, and group RAME4 received 200 μg/kg LPS plus 4 mg/kg RAME. Each group had two subgroups: the 3rd and 24th hr. The eye tissues were collected and investigated biomicroscopically (clinical manifestations and scoring, molecularly(qRT-PCR analyses of tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and caspase 3 and caspase 9 mRNA expression), biochemically (Superoxide dismutase activity (SOD), Glutathione (GSH), and malondialdehyde levels (MDA)) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Results: Melatonin receptor agonist treatment reduced the clinical score count of ocular inflammation in the uveitic rats. TNF-α, VEGF, caspase 9, and caspase 3 levels markedly decreased in the uveitic rats. Melatonin receptor agonists significantly ameliorated fixed changes in GSH, SOD, and MDA levels. Melatonin receptor agonists also ameliorated histopathological injury in eye tissues associated with uveitis. Conclusion: Melatonin receptor agonists ameliorated the inflammatory response in EIU. These findings suggest that melatonin receptor agonists may represent a potential novel therapeutic drug for uveitis treatment.
Yazarlar (6)
1
Tugba Nurcan Yuksel
2
Muhammed Yayla
ORCID: 0000-0002-0659-3084
3
Duygu Kose
4
Zekai Halici
5
Erdinc Bozkurt
6
Erdem Toktay
ORCID: 0000-0002-0715-2707
Anahtar Kelimeler
Lipopolysaccharides
Melatonin
Oxidative stress
Rats
Uveitis
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Ataturk University, Faculty of Medicine
Erzurum Turkey
Kafkas Üniversitesi
Kars Turkey
Kahramanmaras Sütçü Imam Üniversitesi
Kahramanmaras Turkey
Tekirdağ Namık Kemal Üniversitesi
Tekirdag Turkey
University of Health Sciences
Istanbul Turkey
Metrikler
2
Atıf
6
Yazar
5
Anahtar Kelime