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Flask and reactor scale production of plant growth regulators by Inonotus hispidus: optimization, immobilization and kinetic parameters

Preparative Biochemistry and Biotechnology · Ocak 2023

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YÖKSİS Kayıtları
Flask and reactor scale production of plant growth regulators by Inonotus hispidus: optimization, immobilization and kinetic parameters
Informa UK Limited · 2023 SCI-Expanded
PROFESÖR HASAN HÜSEYİN DOĞAN →
Flask and reactor scale production of plant growth regulators by Inonotus hispidus : optimization, immobilization and kinetic parameters
Prep Biochem Biotechnol . · 2023 SCI-Expanded
PROFESÖR HASAN HÜSEYİN DOĞAN →

Makale Bilgileri

DergiPreparative Biochemistry and Biotechnology
Yayın TarihiOcak 2023
Cilt / Sayfa53 · 1210-1223
Özet The aims of the presented study are to compare submerged, static, and solid-state fermentation in the production of gibberellic acid (GA3), indole acetic acid (IAA), and abscisic acid (ABA) by Inonotus hispidus, to optimize with a statistical approach, and to determine the kinetic parameters under flask and reactor conditions. The maximum concentrations of GA3, (2478.85 ± 68.53 mg/L), ABA, (273.26 ± 6.17 mg/L) and IAA (30.67 ± 0.19 mg/L) were obtained in submerged conditions. After optimization, these values reached 2998.85 ± 28.85, 339.47 ± 5.50, and 34.56 ± 0.25 mg/L, respectively. Immobilization of fungal cells on synthetic fiber, polyurethane foam, and alginate beads resulted in an increase in plant growth regulators (PGR) production by 5.53%− 5.79% under optimized conditions. At the reactor scale, a significant increase was observed for GA3 concentration, 5441.54 mg/L, which was 2.14 and 1.45 times higher than non-optimized and optimized conditions in the flask scale, respectively. The maximum values for ABA and IAA were 390.39 and 44.79 mg/L, respectively. Although the specific growth rate (µ) decreases relatively from non-optimized flask conditions to optimized reactor conditions, it was observed that the PGR amounts produced per liter medium (rp) and per gram biomass (Qp) increased significantly. This is the first report on the synthesis of PGR by Inonotus hispidus which could be crucial for sustainable agriculture.

Yazarlar (8)

1
Bilal Doğan
2
Zeki Yıldız
3
Nilüfer Aksöz
4
Ayşe Betül Eninanç
5
İlknur Dağ
6
Abdunnasır Yıldız
7
Hasan Huseyin Dogan
8
Mustafa Yamaç

Anahtar Kelimeler

Abscisic acid gibberellic acid indole acetic acid Inonotus hispidus optimization

Kurumlar

Dicle University, Faculty of Medicine
Diyarbakir Turkey
Ena Laboratories
Ankara Turkey
Eskişehir Osmangazi Üniversitesi
Eskisehir Turkey
Hacettepe Üniversitesi
Ankara Turkey
Ileri District
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

3
Atıf
8
Yazar
5
Anahtar Kelime