Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Discrimination of viable and dead Vibrio parahaemolyticus subjected to low temperatures using Propidium Monoazide – Quantitative loop mediated isothermal amplification (PMA-qLAMP) and PMA-qPCR
Microbial Pathogenesis · Temmuz 2019
YÖKSİS Kayıtları
Discrimination of viable and dead Vibrio parahaemolyticus subjected to low temperatures using Propidium Monoazide – Quantitative loop mediated isothermal amplification (PMA-qLAMP) and PMA-qPCR
Microbial Pathogenesis · 2019 SCI
DOÇENT ARİFE EZGİ TELLİ →
Discrimination of viable and dead Vibrio parahaemolyticus subjected to low temperatures using Propidium Monoazide Quantitative loop mediated isothermal amplification (PMA-qLAMP) and PMA-qPCR
Microbial Pathogenesis · 2019 SCI-Expanded
DOÇENT ARİFE EZGİ TELLİ →
Discrimination of viable and dead Vibrio parahaemolyticus subjected to low temperatures using Propidium Monoazide Quantitative loop mediated isothermal amplification (PMA-qLAMP) and PMA-qPCR
Microbial Pathogenesis · 2019 SCI-Expanded
PROFESÖR YUSUF DOĞRUER →
Makale Bilgileri
DergiMicrobial Pathogenesis
Yayın TarihiTemmuz 2019
Cilt / Sayfa132 · 109-116
Scopus ID2-s2.0-85065030659
Erişim🔓 Açık Erişim
Özet
The aim of this study was to determine the effect of cold (4 °C) and subzero (−18 °C, −45 °C) temperatures on the occurrence time of membrane damage to provide Propidium Monoazide (PMA) penetration of Vibrio parahaemolyticus inoculated to the sea bass. Direct plate counting (DPC) and PMA-based quantitative loop-mediated isothermal amplification (qLAMP) and qPCR was utilized for discrimination of dead and live bacteria on the designated storage days (1, 3, 7, and 14). The optimum amount of PMA was 50 μM for inhibition of amplification derived from dead cells in spiked samples. The number of live V. parahaemolyticus was detectable at the end of the 14. day using PMA-qLAMP and PMA-qPCR at all the temperatures. On the 7th day, culturability has lost at any of the storage temperatures and DPCs at −18 °C and −45 °C revealed a difference of about 1 log 10 CFU/ml between 1st and 3rd days. The same difference was also observed in PMA–qLAMP and PMA–qPCR on the same days (0.59–0.95 log 10 CFU/ml). Subzero temperatures have the highest rate of viability while causing the fastest decrease in culturability in sample groups as a result of the higher level of transition to VBNC state. qLAMP and qPCR methods in the PMA-treated and nontreated groups on the storage days at all temperatures gave similar results (p > 0.05).
Yazarlar (2)
1
A. Ezgi Telli
2
Y. Doğruer
Anahtar Kelimeler
Loop-mediated isothermal amplification
Membrane damage
PMA-qPCR
Propidium monoazide
Subzero temperatures
Viable and dead bacteria
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
20
Atıf
2
Yazar
6
Anahtar Kelime