Scopus
YÖKSİS Eşleşti
Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen
Cell Journal · Mart 2019
YÖKSİS Kayıtları
Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen.
Cell Journal · 2019 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen.
Cell Journal · 2019 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →
Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen.
Cell Journal · 2019 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Makale Bilgileri
DergiCell Journal
Yayın TarihiMart 2019
Cilt / Sayfa21 · 7-13
Scopus ID2-s2.0-85057041328
Özet
Objective: The purpose of the present study was to assess the effects of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Materials and Methods: In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, the ejaculates were diluted in base extender contained ellagic acid at 0 (control), 0.5, 1, and 2 mM. In experiment 2, ebselen at 0 (control), 10, 20, and 40 μM were added to the extender. Sperm motility, viability, mitochondrial membrane potential, DNA integrity, lipid peroxidation (LPO), the antioxidant potential (AOP), and total glutathione (tGSH) were evaluated at 0, 24, 48, and 72 hours of preservation. Results: Supplementation of ellagic acid at 1 and 2 mM resulted in higher sperm motility and viability at 0 hours of storage. Ellagic acid at 2 mM led to higher motility and viability compared to controls after 0, 24, and 48 hours of preservation and increased AOP after 24 and 72 hours. Higher tGSH was at 1 mM ellagic acid, compared to control after 72 hours. Addition of ebselen at a concentration of 40 μM increased motility at 24 and 48 hours and 10 μM produced the same effect after 48 and 72 hours of storage as well as higher viability, compared to the controls after 0 hours of storage. Sperm DNA integrity was significantly improved after 24, 48, and 72 hours with the addition of ebselen at 10 μM, and after 72 hours at 40 μM. Addition of 40 mM ebselen also reduced the LPO levels after 24 hours of storage compared to the controls. Conclusion: The results showed that supplementation of ellagic acid and ebselen in semen extender has a potential effect on sperm and oxidative stress parameters during liquid preservation of ram semen.
Yazarlar (8)
1
Mustafa Numan Bucak
2
Mustafa Bodu
ORCID: 0000-0002-8432-7347
3
N. Baspinar
4
Şükrü Güngör
5
Pinar Ili
6
Begimay Acibaeva
7
Tohid Rezaei Topraggaleh
8
Şükrü Dursun
Anahtar Kelimeler
Ebselen
Ellagic acid
Preservation
Ram
Sperm parameters
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur Turkey
Pamukkale Üniversitesi
Denizli Turkey
Royan Institute
Tehran Iran
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
14
Atıf
8
Yazar
5
Anahtar Kelime