Scopus
YÖKSİS DOI Eşleşti
SJR Q2
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
Cryobiology · Ağustos 2020
YÖKSİS Kayıtları
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
Cryobiology · 2020 SCI-Expanded
Dr. Öğr. Üyesi MUSTAFA BODU →
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
CRYOBIOLOGY · 2020 SCI-Expanded
Dr. Öğr. Üyesi MUSTAFA BODU →
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
Cryobiology · 2020 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
Cryobiology · 2020 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Influence of fetuin and hyaluronan on the post thaw quality and fertilizing ability of Holstein bull semen
2015 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
The influence of cysteine and taurine on microscopic oxidative stress parameters and fertilizing ability of bull semen following cryopreservation
2009 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effects of antioxidants on post thawed bovine sperm and oxidative stress parameters Antioxidants protect DNA integrity against cryodamage
2010 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
The effect of cysteine and glutathione on sperm and oxidative stress parameters of post thawed bull semen
2010 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
The effect of raffinose and methionine on frozen thawed Angora buck Capra hircus ancryrensis semen quality lipid peroxidation and antioxidant enzyme activities
2010 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effects of cysteine and ergothioneine on post thawed Merino ram sperm and biochemical parameters
2011 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze thaw process
2013 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Influence of various antioxidants added to TCM 199 on post thaw bovine sperm parameters DNA integrity and fertilizing ability
2014 ISSN: 00112240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effects of antioxidants on post thawed bovine sperm and oxidative stress parameters Antioxidants protect DNA integrity against cryodamage
2010 ISSN: 00112240 SCI-Expanded 7 atıf
Prof. Dr. NURİ BAŞPINAR →
Effects of cysteine and ergothioneine on post thawed Merino ram sperm and biochemical parameters
2011 ISSN: 00112240 SCI-Expanded 3 atıf
Prof. Dr. NURİ BAŞPINAR →
Raffinose and hypotaurine improve the post thawed Merino ram sperm parameters
2013 ISSN: 00112240 SCI-Expanded
Prof. Dr. AHMED NURİ KURŞUNLU →
Raffinose and hypotaurine improve the postthawed Merino ram sperm parameters
2013 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
Effects of dithioerythritol on ram semen after the freeze thawingprocess
2011 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
Raffinose and Hypotaurine Improve the Post-Thawed Merino Ram Sperm Parameters
2013 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Methionine supplementation improves ram sperm parameters during liquid storage at 5 degrees C
2012 ISSN: 0011-2240 SCI
Prof. Dr. MUSTAFA NUMAN BUCAK →
The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4°C
2012 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
2020 ISSN: 0011-2240 SCI-Expanded Q2
Dr. Öğr. Üyesi MUSTAFA BODU →
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
2020 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
2020 ISSN: 0011-2240 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
2020 ISSN: 0011-2240 SCI-Expanded Q2
Dr. Öğr. Üyesi MUSTAFA BODU →
Makale Bilgileri
Dergi
Cryobiology
ISSN00112240
Yayın TarihiAğustos 2020
Cilt / Sayfa95 · 157-163
Scopus ID2-s2.0-85082852665
Özet
The objective of this study was to compare the effects of different concentrations of two different cryoprotectants (glycerol, G and ethylene glycol, EG) and trehalose (T), added to the semen extender, on post-thaw ram sperm parameters. Ejaculates, collected from 6 Merino rams, were pooled and evaluated at 37 °C. The pooled samples were divided into six equal aliquots, and diluted in Tris-based extenders containing 5% G, 3% G + 60 Mm T, 1.5% G + 100 Mm T, 5% EG, 3% EG + 60 mM T, and 1.5% EG + 100 Mm T. Subsequently, the samples were cooled to 5 °C, frozen in 0.25-ml French straws, and stored in liquid nitrogen (LN2). Frozen samples were thawed individually, at 37 °C for 25 s in a water bath, for evaluation. Sperm motility was assessed using a phase-contrast microscope with a warm stage maintained at 37 °C. Acrosome integrity (FITC/PNA-PI), sperm viability (SYBR-14/PI), mitochondrial activity (JC-1/PI), DNA damage (COMET assay) and DNA fragmentation (TUNEL test) were determined. The group of samples diluted in an extender containing 5% of glycerol (Group 5% G) displayed higher percentages of subjective motility, viability and mitochondrial activity of sperm, compared to the other groups (P < 0.05). On the other hand, Group 3% G + 60 mM T yielded the second-best results for subjective motility, viability and mitochondrial activity of sperm, when compared to the other groups. The post-thaw sperm parameters of Group 3% G + 60 Mm T did not show any statistically significant difference from those of Group 5% G. There were no statistically significant differences between the groups for acrosome integrity (P > 0.05). The results of the COMET assay showed that the use of low concentrations of cryoprotectants in combination with trehalose decreased sperm DNA damage. Accordingly, Group 1.5% G + 100 mM T and Group 3% EG + 60 mM T benefited from a significantly stronger cryoprotective effect on DNA integrity, in comparison to Group 5% G (P < 0.05). According to the results of the TUNEL test, the combined use of low concentrations of cryoprotectants with trehalose decreased sperm DNA damage, when compared to the use of 5% glycerol, but this difference was statistically insignificant (P > 0.05). In conclusion, G and EG concentrations can be reduced by adding various amounts of T (60 mM, 100 mM) to the semen extender. The addition of 5% of glycerol and 3% G + 60 mM T to the semen extender did not yield statistically different post-thaw sperm parameters, when compared for protection against cryoinjury. Post-thaw sperm parameters can be improved by the supplementation of the semen extender with 3% G + 60 mM T. Thus, we recommend the use of freezing extenders containing low cryoprotectant concentrations (3% G) combined with trehalose to avoid the high level of toxic and osmotic damage caused by 5% G.
Yazarlar (11)
1
Ali Erdem Öztürk
2
Mustafa Bodu
ORCID: 0000-0002-8432-7347
3
Mustafa Numan Bucak
4
Vahit Ağır
5
Ayşe Özcan
6
Nazan Keskin
7
Pinar Ili
8
Tohid Rezaei Topraggaleh
9
Hümeyra Sidal
10
N. Baspinar
11
Şükrü Dursun
Anahtar Kelimeler
Ethylene glycol
Glycerol
Ram semen
Sperm parameters
Trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Ankara Yildirim Beyazit University
Ankara Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Tabriz University of Medical Sciences
Tabriz Iran
Scimago Dergi (ISSN Eşleşmesi)
Cryobiology
Q2
SJR Skoru0,519
H-Index98
YayıncıAcademic Press Inc.
ÜlkeUnited States
Agricultural and Biological Sciences (miscellaneous) (Q2)
Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q2)
Medicine (miscellaneous) (Q3)
Metrikler
46
Atıf
11
Yazar
5
Anahtar Kelime