Scopus
YÖKSİS Eşleşti
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
Cryobiology · Ekim 2020
YÖKSİS Kayıtları
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
Cryobiology · 2020 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
Cryobiology · 2020 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
Cryobiology · 2020 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
Cryobiology · 2020 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiCryobiology
Yayın TarihiEkim 2020
Cilt / Sayfa96 · 19-29
Scopus ID2-s2.0-85090751385
Özet
This study aimed to evaluate the comparative effects of taxifolin hydrate and trehalose on the quality of frozen-thawed ram spermatozoa for the first time. Ejaculates collected from six mature rams were pooled, and divided to eight equal aliquots to extend them with different concentrations of glycerol (%5 and %3), taxifolin hydrate (10, 100, and 500 μM), and trehalose (60 mM) as eight groups (G5T0, G5T10, G5T100, G5T500, G3T0, G3T10, G3T100, and G3T500). After freeze-thawing process of cryopreservation, microscopic and oxidative stress parameters, and gene expression levels were investigated for understanding of possible impacts of taxifolin hydrate and trehalose. The study showed that G3T10 resulted in the highest post-thawed viability and mitochondrial activity. Moreover, all extenders with taxifolin hydrate reduced DNA fragmentation in comparison to G5T0, but DNA damage was prevented at the highest rate in presence of G5T10. The level of LPO significantly decreased in the groups G5T500 and G3T100, and the expression levels of NQO1, GCLC, and GSTP1 genes significantly increased in the groups G5T100, G5T500, G3T10, and G3T100 compared to the group G5T0. Finally, co-supplementation of tris-based extender having 3% glycerol with 60 mM trehalose and 10 μM taxifolin hydrate in cryopreservation extender may be recommended to improve the quality of post-thawed ram spermatozoa. However, further in vivo and in vitro studies are suggested to evaluate fertility rates of frozen-thawed ram spermatozoa co-supplemented with trehalose and taxifolin hydrate.
Yazarlar (11)
1
Mustafa Numan Bucak
2
Nazan Keskin
3
Pinar Ili
4
Mustafa Bodu
ORCID: 0000-0002-8432-7347
5
Pınar Peker Akalin
6
Ali Erdem Öztürk
7
Hüseyin Özkan
8
Tohid Rezaei Topraggaleh
9
Fikret Sari
10
N. Baspinar
11
Şükrü Dursun
Anahtar Kelimeler
Antioxidants
Cryopreservation
Gene expression
Oxidative stress
Ram semen
Sperm parameters
Taxifolin hydrate
Trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Mustafa Kemal Üniversitesi
Antakya Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Tabriz University of Medical Sciences
Tabriz Iran
Metrikler
21
Atıf
11
Yazar
8
Anahtar Kelime