Scopus
YÖKSİS Eşleşti
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · Ekim 2020
YÖKSİS Kayıtları
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →
Makale Bilgileri
DergiBiopreservation and Biobanking
Yayın TarihiEkim 2020
Cilt / Sayfa18 · 441-448
Scopus ID2-s2.0-85092681301
Özet
Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from Konya Merino rams were pooled and diluted with a tris-based extender containing additives, including 5% glycerol, 3% glycerol +60 mM trehalose, 1.5% glycerol +100 mM trehalose, 5% ethylene glycol, 3% ethylene glycol +60 mM trehalose, and 1.5% ethylene glycol +100 mM trehalose. They were all cooled to 5°C and then frozen in 0.25 mL French straws in liquid nitrogen. The samples were thawed at 37°C and centrifuged to remove the diluents. Then, they were processed using a scanning transmission electron microscope. In the statistical analysis, the number of ultrastructurally cryodamaged and intact spermatozoa were counted in longitudinal and transverse ultrathin sections in all groups by electron microscopic examination. The amount of intact spermatozoa in the groups containing 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose was found to be higher than other groups (p < 0.05). As a result, it was suggested that the groups of 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of frozen-thawed Konya Merino ram spermatozoa among the groups.
Yazarlar (8)
1
Nazan Keskin
2
Cennet Erdogan
3
Mustafa Numan Bucak
4
Ali Erdem Öztürk
5
Mustafa Bodu
ORCID: 0000-0002-8432-7347
6
Pinar Ili
7
N. Baspinar
8
Şükrü Dursun
Anahtar Kelimeler
cryopreservation
electron microscopy
ethylene glycol
glycerol
Konya Merino ram spermatozoa
trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
23
Atıf
8
Yazar
6
Anahtar Kelime