Scopus
YÖKSİS DOI Eşleşti
SJR Q3
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · Ekim 2020
YÖKSİS Kayıtları
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
Dr. Öğr. Üyesi MUSTAFA BODU →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
Dr. Öğr. Üyesi MUSTAFA BODU →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
Prof. Dr. NURİ BAŞPINAR →
Cryopreservation Effects on Ram Sperm Ultrastructure
Biopreservation and Biobanking · 2020 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm
2021 ISSN: 1947-5535 SCI-Expanded Q3
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effect of Bovine Serum Albumin Supplementation in Tris-Soybean Lecithin-Based Extender on Quality of Chilled Ram Epididymal Spermatozoa
2021 ISSN: 1947-5535 SCI-Expanded Q3
Prof. Dr. MUSTAFA NUMAN BUCAK →
The effects of silymarin supplementation on post-thawed human sperm functional parameters
2023 ISSN: 1947-5535 SCI-Expanded Q3
Arş. Gör. SEDA ŞİMŞEK →
The Effects of Silymarin Supplementation on Post-Thawed Human Sperm Functional Parameters
2022 ISSN: 1947-5535 SCI-Expanded Q3
Arş. Gör. SEDA ŞİMŞEK →
Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze–Thaw Process
2024 ISSN: 1947-5535 SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Makale Bilgileri
ISSN19475535
Yayın TarihiEkim 2020
Cilt / Sayfa18 · 441-448
Scopus ID2-s2.0-85092681301
Özet
Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from Konya Merino rams were pooled and diluted with a tris-based extender containing additives, including 5% glycerol, 3% glycerol +60 mM trehalose, 1.5% glycerol +100 mM trehalose, 5% ethylene glycol, 3% ethylene glycol +60 mM trehalose, and 1.5% ethylene glycol +100 mM trehalose. They were all cooled to 5°C and then frozen in 0.25 mL French straws in liquid nitrogen. The samples were thawed at 37°C and centrifuged to remove the diluents. Then, they were processed using a scanning transmission electron microscope. In the statistical analysis, the number of ultrastructurally cryodamaged and intact spermatozoa were counted in longitudinal and transverse ultrathin sections in all groups by electron microscopic examination. The amount of intact spermatozoa in the groups containing 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose was found to be higher than other groups (p < 0.05). As a result, it was suggested that the groups of 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of frozen-thawed Konya Merino ram spermatozoa among the groups.
Yazarlar (8)
1
Nazan Keskin
2
Cennet Erdogan
3
Mustafa Numan Bucak
4
Ali Erdem Öztürk
5
Mustafa Bodu
ORCID: 0000-0002-8432-7347
6
Pinar Ili
7
N. Baspinar
8
Şükrü Dursun
Anahtar Kelimeler
cryopreservation
electron microscopy
ethylene glycol
glycerol
Konya Merino ram spermatozoa
trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Biopreservation and Biobanking
Q3
SJR Skoru0,372
H-Index46
YayıncıMary Ann Liebert Inc.
ÜlkeUnited States
Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q3)
Medicine (miscellaneous) (Q3)
Cell Biology (Q4)
Metrikler
27
Atıf
8
Yazar
6
Anahtar Kelime