Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze-thawed ram spermatozoa
Andrology · Mayıs 2021
YÖKSİS Kayıtları
Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa
Andrology · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze\u2010thawed ram spermatozoa
Andrology · 2021 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa
Wiley · 2021 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →
Makale Bilgileri
DergiAndrology
Yayın TarihiMayıs 2021
Cilt / Sayfa9 · 1000-1009
Scopus ID2-s2.0-85100113093
Erişim🔓 Açık Erişim
Özet
Background: Freeze-thawing process negatively affects ram spermatozoa in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of spermatozoa during freeze-thawing is suggested to improve sperm parameters. Objectives: The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant parameters, antioxidant-related gene expressions and DNA integrity during the freeze-thawing process, in low glycerol concentration. Methods: Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris-based extender containing different concentrations of glycerol (G5; %5 and G3; %3), fetuin (F; 2.5, 5 and 15 mg/mL) and trehalose (60 mm) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5 and G3F15). Results: G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p < 0.05). Also, G3F0 displayed considerably more cryoprotective effect compared with G5F0 group (p < 0.05) in terms of motility, mitochondrial activity and viability rates. Lipid peroxidation levels decreased in G5F5 group compared with G5F0 group (p < 0.05). The levels of total glutathione increased in G3F2.5 group (p < 0.05) in comparison with the G5F0 group. NQO1 gene levels were upregulated approximately twofold in G5F5, G5F15, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). The levels of GCLC gene were approximately twofold higher in G3F0, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). GSTP1 gene levels were significantly higher with different levels in all treatment groups except for G5F2.5 and G3F0 groups in comparison with G5F0 group (p < 0.05). Conclusions: Co-supplementation of tris-based extender having low glycerol (3%) with trehalose and fetuin to enhance the quality of ram spermatozoa after freeze-thawing process is recommended.
Yazarlar (11)
1
Mustafa Numan Bucak
2
Pınar Peker Akalin
3
Nazan Keskin
4
Mustafa Bodu
ORCID: 0000-0002-8432-7347
5
Ali Erdem Öztürk
6
Pinar Ili
7
Hüseyin Özkan
8
Tohid Rezaei Topraggaleh
9
Halil Ozancan Arslan
10
N. Baspinar
11
Şükrü Dursun
Anahtar Kelimeler
fetuin
freeze-thawing
glycerol
ram spermatozoa
trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Erciyes Üniversitesi
Kayseri Turkey
Mustafa Kemal Üniversitesi
Antakya Turkey
Pamukkale Üniversitesi
Denizli Turkey
Pamukkale Üniversitesi Tip Fakültesi
Denizli Turkey
School of Medicine
Urmia Iran
Selçuk Üniversitesi
Selçuklu Turkey
T.C. Tarim ve Köyişleri Bakanliği
Antakya Turkey
Metrikler
10
Atıf
11
Yazar
5
Anahtar Kelime