Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
The Effects of Different Doses of ROCK Inhibitor, Antifreeze Protein III, and Boron Added to Semen Extender on Semen Freezeability of Ankara Bucks
Molecules · Kasım 2022
YÖKSİS Kayıtları
The Effects of Different Doses of ROCK Inhibitor, Antifreeze Protein III, and Boron Added to Semen Extender on Semen Freezeability of Ankara Bucks
Molecules · 2022 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
The Effects of Different Doses of ROCK Inhibitor, Antifreeze Protein III, and Boron Added to Semen Extender on Semen Freezeability of Ankara Bucks
MDPI AG · 2022 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Makale Bilgileri
DergiMolecules
Yayın TarihiKasım 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85142655521
Erişim🔓 Açık Erişim
Özet
In the presented study, the effects of ROCK inhibitor Y-27632, antifreeze protein III, and boron at two different doses were investigated on the spermatological parameters of Ankara buck semen after freeze–thawing. Ejaculates were collected from bucks using an electroejaculator during the breeding season. The ejaculates that showed appropriate characteristics were pooled and used in the dilution and freezing of semen. The extender groups were formed by adding two different doses of three different additives (ROCK inhibitor Y-27632, 5 and 20 µM; antifreeze protein III, 1 and 4 µg/mL; boron, 0.25 and 1 mM) to the control extender. The semen was diluted with the different extenders at 35–37 °C and loaded into straws. Sperm samples frozen in liquid nitrogen vapors, following equilibration, were stored in liquid nitrogen. It was observed that extender supplementation improved post-thaw motility of Ankara buck semen after freeze–thawing. Differences were significant (p < 0.01) for 5 and 10 µM doses of ROCK inhibitor (71.82% and 74.04 % motility), as well as for 0.25 and 1 mM doses of boron (76.36% and 72.08% motility), compared to the control group (66.15% motility). With respect to the evaluation of acrosomal integrity and mitochondrial activity after freeze–thawing, although supplementation provided protection at all doses, the efficacy was not statistically significant (p > 0.05). It was observed that DNA damage was improved by antifreeze protein III at 1 µg/mL (1.23% ± 0.23%) and by boron at all doses (0.25 mM: 1.83% and 1 mM: 1.18%) compared to the control group (3.37%) (p < 0.01), following the thawing process. In the present study, it was determined that some additives added to the extender provided significant improvements in buck spermatozoa motility and DNA damage after thawing.
Yazarlar (6)
1
Ömer Faruk Karaşör
2
Mustafa Numan Bucak
3
Mihai Cenariu
4
Mustafa Bodu
ORCID: 0000-0002-8432-7347
5
M. Taşpinar
6
Filiz Taşpınar
Anahtar Kelimeler
Ankara buck
antifreeze protein III
boron
ROCK inhibitor
semen freezing
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Tarimsal Araştirmalar ve Politikalar Genel Müdürlüğü, Ankara
Ankara Turkey
Universitatea de Medicina si Farmacie Iuliu Hatieganu din Cluj-Napoca
Cluj Napoca Romania
Metrikler
4
Atıf
6
Yazar
5
Anahtar Kelime