Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Functionalization of whole-cell bacterial reporters with magnetic nanoparticles
Microbial Biotechnology · Ocak 2011
YÖKSİS Kayıtları
Functionalization of whole cell bacterial reporters with magnetic nanoparticles
MICROBIAL BIOTECHNOLOGY · 2011 SCI-Expanded 31 atıf
PROFESÖR MUSTAFA ÖZMEN →
Makale Bilgileri
DergiMicrobial Biotechnology
Yayın TarihiOcak 2011
Cilt / Sayfa4 · 89-97
Scopus ID2-s2.0-79951883430
Erişim🔓 Açık Erişim
Özet
We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response to salicylate, toluene/xylene and alkanes, were functionalized with 18±3nm iron oxide MNPs to acquire magnetic function. The efficiency of MNPs functionalization of Acinetobacter bioreporters was 99.96±0.01%. The MNPs-functionalized bioreporters (MFBs) can be remotely controlled and collected by an external magnetic field. The MFBs were all viable and functional as good as the native cells in terms of sensitivity, specificity and quantitative response. More importantly, we demonstrated that salicylate sensing MFBs can be applied to sediments and garden soils, and semi-quantitatively detect salicylate in those samples by discriminably recovering MFBs with a permanent magnet. The magnetically functionalized cells are especially useful to complex environments in which the indigenous cells, particles and impurities may interfere with direct measurement of bioreporter cells and conventional filtration is not applicable to distinguish and harvest bioreporters. The approach described here provides a powerful tool to remotely control and selectively manipulate MNPs-functionalized cells in water and soils. It would have a potential in the application of environmental microbiology, such as bioremediation enhancement and environment monitoring and assessment. © 2010 The Authors. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
Yazarlar (8)
1
Dayi Zhang
2
Rawil F. Fakhrullin
3
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
4
Hui Wang
5
Jian Wang
6
Vesselin N. Paunov
7
Guanghe Li
8
Wei E. Huang
Kurumlar
BGI-Shenzhen
Shenzhen China
Kazan Federal University
Kazan Russian Federation
Selçuk Üniversitesi
Selçuklu Turkey
The University of Sheffield
Sheffield United Kingdom
Tsinghua University
Beijing China
UK Centre for Ecology & Hydrology
Wallingford United Kingdom
University of Hull
Hull United Kingdom
Metrikler
87
Atıf
8
Yazar