Scopus
YÖKSİS Eşleşti
Binding affinity of serum proteins to epoxy modified magnetite nanoparticles
Advanced Science Letters · Ekim 2012
YÖKSİS Kayıtları
Binding Affinity of Serum Proteins to Epoxy Modified Magnetite Nanoparticles
ADVANCED SCIENCE LETTERS · 2012 SCOPUS, Chemical Abstracts, Elsevier Bibliographic Databases, Compendex, Biological Sciences Abstracts, Biotechnology and BioEngineering Abstracts, Biotechnology Research Abstracts, Bacteriology Abstracts (Microbiology B), Neurosciences Abstracts, Engineering Research Database, Technology Research Database, Environmental Science and Pollution Management 8 atıf
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Makale Bilgileri
DergiAdvanced Science Letters
Yayın TarihiEkim 2012
Cilt / Sayfa17 · 143-148
Scopus ID2-s2.0-84870945939
Özet
Superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. SPIONs modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane, which resulted in the presence of a high population of epoxy groups on the particles surface. The surface functional SPIONs can be bound with active bio-substance, and have a wide application prospect in the fields of biology and medicine. The binding percentage of proteins was studied with epoxy modified SPIONs. Several serum proteins including albumin, α-globulin, hemoglobin, cytochrome c and myoglobin were studied for immobilization. Epoxy terminated SPIONs were shown the highest binding ability for immobilization of cytochrome c and hemoglobin with 1 and 0.86 mg total protein at a particle concentration of 20 mg/mL among proteins, respectively. This binding ability was also shown by using sodium dodecylsulfate polyacrylamide gel electrophoresis technique (SDS-PAGE). Protein attachments were also examined by Scanning Electron Microscopy (SEM). © 2012 American Scientific Publishers. All rights reserved.
Yazarlar (4)
1
Esra Maltas Cagil
2
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
3
Salih Yildiz
4
Mustafa Ersöz
Anahtar Kelimeler
Cytochrome c
Epoxy
Hemoglobine
Magnetic nanoparticles
SDS-PAGE
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
4
Yazar
5
Anahtar Kelime