Scopus
YÖKSİS Eşleşti
Novel humic acid-bonded magnetite nanoparticles for protein immobilization
Materials Science and Engineering C · Eylül 2014
YÖKSİS Kayıtları
Novel Humic Acid Bonded Magnetite Nanoparticles for Protein Immobilization
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS · 2014 SCI-Expanded 5 atıf
PROFESÖR MUSTAFA ÖZMEN →
Novel humic acid bonded magnetite nanoparticles for protein immobilization
Materials Science and Engineering: C · 2014 SCI-Expanded
PROFESÖR ESRA MALTAŞ ÇAĞIL →
Novel humic acid bonded magnetite nanoparticles for protein immobilization
Materials Science and Engineering: C · 2014 SCI-Expanded
PROFESÖR SALİH ZEKİ BAŞ →
Makale Bilgileri
DergiMaterials Science and Engineering C
Yayın TarihiEylül 2014
Cilt / Sayfa42 · 546-552
Scopus ID2-s2.0-84903139028
Özet
The present paper is the first report that introduces (i) a useful methodology for chemical immobilization of humic acid (HA) to aminopropyltriethoxysilane-functionalized magnetite iron oxide nanoparticles (APS-MNPs) and (ii) human serum albumin (HSA) binding to the obtained material (HA-APS-MNPs). The newly prepared magnetite nanoparticle was characterized by using Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and elemental analysis. Results indicated that surface modification of the bare magnetite nanoparticles (MNPs) with aminopropyltriethoxysilane (APS) and HA was successfully performed. The protein binding studies that were evaluated in batch mode exhibited that HA-APS-MNPs could be efficiently used as a substrate for the binding of HSA from aqueous solutions. Usually, recovery values higher than 90% were found to be feasible by HA-APS-MNPs, while that value was around 2% and 70% in the cases of MNPs and APS-MNPs, respectively. Hence, the capacity of MNPs was found to be significantly improved by immobilization of HA. Furthermore, thermal degradation of HA-APS-MNPs and HSA bonded HA-APS-MNPs was evaluated in terms of the Horowitz-Metzger equation in order to determine kinetic parameters for thermal decomposition. Activation energies calculated for HA-APS-MNPs (20.74 kJ mol- 1) and HSA bonded HA-APS-MNPs (33.42 kJ mol- 1) implied chemical immobilization of HA to APS-MNPs, and tight interactions between HA and HA-APS-MNPs. © 2014 Elsevier B.V.
Yazarlar (5)
1
Mevlut Bayrakci
2
Orhan Gezici
3
Salih Zeki Bas
ORCID: 0000-0003-2822-8851
4
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
5
Esra Maltas Cagil
Anahtar Kelimeler
Horowitz-Metzger method
Human serum albumin (HSA)
Humic substances
Magnetite nanoparticle
Protein binding
Kurumlar
Niğde Ömer Halisdemir University
Nigde Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
22
Atıf
5
Yazar
5
Anahtar Kelime