Scopus
YÖKSİS Eşleşti
Effective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathway
Investigational New Drugs · Nisan 2023
YÖKSİS Kayıtları
Effective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathway
INVESTIGATIONAL NEW DRUGS · 2023 SCI-Expanded
PROFESÖR AHMED NURİ KURŞUNLU →
Effective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathway
Investigational New Drugs · 2023 SCI-Expanded
PROFESÖR MUSTAFA ÖZMEN →
Makale Bilgileri
DergiInvestigational New Drugs
Yayın TarihiNisan 2023
Cilt / Sayfa41 · 202-209
Scopus ID2-s2.0-85149730205
Özet
This study aimed to evaluate the possible anticancer effects of two different pillar[5]arene derivatives (5Q-[P5] and 10Q-P[5]) on two different pancreatic cancer cell lines in vitro. For this purpose, changes in the expression of major genes that play a role in apoptosis and caspase pathways were investigated. Panc-1 and BxPC-3 cell lines were used in the study and the cytotoxic dose of pillar[5]arenes was determined by the MTT method. Changes in gene expression after pillar[5]arenes treatment were evaluated by real-time polymerase chain reaction (qPCR). Apoptosis was studied by flow cytometry. As a result of analysis, it was determined that proapoptotic genes and genes involved in major caspase activation were upregulated and antiapoptotic genes were down-regulated in Panc-1 cell line treated with pillar[5]arenes. Flow cytometric apoptosis analysis also showed an increased apoptosis rate in this cell line. On the contrary, although MTT analysis showed cytotoxic effect in BxPC-3 cell line treated with two pillar[5]arene derivatives, the apoptosis pathway was not active. This suggested that it may activate different death pathways for BxPC-3 cell line. Thus, it was first determined that the pillar[5]arene derivatives reduced cancer cell proliferation on pancreatic cancer cells.
Yazarlar (11)
1
Mehmet Ali Karaselek
2
Serkan Kuccukturk
3
Tugce Duran
ORCID: 0000-0002-7353-4527
4
Ahmed Nuri Kursunlu
5
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
6
Ceren Bozdag
7
Selman Alkan
8
Alper Varman
9
Mehmet Aykut Yıldırım
ORCID: 0000-0001-9580-6004
10
Tevfik Kucukkartallar
11
Celalettin Vatansev
ORCID: 0000-0002-0094-1703
Anahtar Kelimeler
BxPC-3
Panc-1
Pancreas cancer
Pillar[5]aren
Kurumlar
Karamanoğlu Mehmetbey Üniversitesi
Karaman Turkey
Karatay Üniversitesi
Konya Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
5
Atıf
11
Yazar
4
Anahtar Kelime