Scopus
Candida rugosa lipase encapsulated with magnetic sporopollenin: design and enantioselective hydrolysis of racemic arylpropanoic acid esters
Preparative Biochemistry and Biotechnology · Kasım 2018
Makale Bilgileri
DergiPreparative Biochemistry and Biotechnology
Yayın TarihiKasım 2018
Cilt / Sayfa48 · 887-897
Scopus ID2-s2.0-85054575181
Özet
The aim of this study was to prepare the encapsulation of Candida rugosa lipase (CRL) with magnetic sporopollenin. The sporopollenin was covalent immobilized onto magnetic nanoparticles (Fe 3 O 4 ), grafted amino (APTES), or epoxy groups (EPPTMS). CRL was sol-gel encapsulated in the presence of magnetic sporopollenin/Fe 3 O 4 nanoparticles. The influence of activation agents ([3-(2,3-epoxypropoxy) propyl] trimethoxysilane (EPPTMS), (3-Aminopropyl)triethoxysilane (APTES) and pH and thermal stabilities of the biocatalyst were assessed. Experimental data showed the improved catalytic activity at different pH and temperature values. At 60 °C, free lipase lost its initial activity within 80 min of time, although the encapsulated lipases retained their initial activities of about 65% by APTES and 60% by EPPTMS after 120 min of heat treatment at 60 °C. The catalytic properties of the encapsulated lipases were utilized to hydrolysis of racemic aromatic carboxylic acid methyl esters (Naproxen and 2-phenoxypropionic acid). The results show that the sporopollenin-based encapsulated lipase (Fe-A-Spo-E) has greater enantioselectivity and conversion in comparison with the encapsulated lipase without supports (lipase-enc).
Yazarlar (3)
1
Elif Ozyilmaz
2
Kubra Etci
3
Mehmet Sezgin
Anahtar Kelimeler
Encapsulated lipase
enzymatic kinetic resolution
magnetic nanoparticles
silane
sporopollenin
stability
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
10
Atıf
3
Yazar
6
Anahtar Kelime