Scopus
YÖKSİS Eşleşti
Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution
International Journal of Biological Macromolecules · Nisan 2021
YÖKSİS Kayıtları
Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution
International Journal of Biological Macromolecules · 2021 SCI-Expanded
PROFESÖR ELİF ÖZYILMAZ →
Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution
International Journal of Biological Macromolecules · 2021 SCI-Expanded
PROFESÖR MUSTAFA YILMAZ →
Makale Bilgileri
DergiInternational Journal of Biological Macromolecules
Yayın TarihiNisan 2021
Cilt / Sayfa175 · 79-86
Scopus ID2-s2.0-85100530353
Özet
Metal organic frameworks (MOFs) are hybrid organic inorganic materials with unique properties such as well-defined pore structure, extremely high surface area, excellent chemical-thermal stability. MOFs-based constructs have been extensively engineered and used for applications, such as enzyme immobilization for bio-catalysis. To obtained a zeolitic imidazole framework-8 (ZIF-8) for enzyme immobilization, Candida rugosa lipase (CRL) was pretreated with calix [4]arene tetracarboxylic acid (Calix) and reacted with Zn and imidazole by co-precipitation method. The prepared biocomposite was characterized by SEM, EDX, FT-IR, and XRD. The prepared CRL@Calix-ZIF-8 with high encapsulation efficiency showed improved resistance to alkali and thermal conditions. The CRL@Calix-ZIF-8 with the biocatalytic activity was 2-folds higher than that of the CRL@ZIF-8 (without Calix). The free lipase lost its catalytic activity completely at 60 °C after 100 min, while the CRL@Calix-ZIF-8 and CRL@ZIF-8 retained about 84% and 73%. It was found that CRL@Calix-ZIF-8 and CRL@ZIF-8 still retained ~83 and 67% of catalytic activity after its 6th use, respectively. The kinetic resolution of the immobilized lipases was examined for enantioselective hydrolysis of racemic naproxen methyl ester. CRL@Calix-ZIF-8 showed enantioselectivity against the racemic naproxen methyl ester, with E = 183 and 131 compared to the CRL@ZIF-8.
Yazarlar (3)
1
Elif Ozyilmaz
2
Sebahat Ascioglu
3
Mustafa Yilmaz
Anahtar Kelimeler
Biocatalysis
Calix
Enantioselective hydrolysis
Lipase
ZIF-8
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
34
Atıf
3
Yazar
5
Anahtar Kelime