Scopus
YÖKSİS Eşleşti
Preparation of regenerable magnetic nanoparticles for cellulase immobilization: Improvement of enzymatic activity and stability
Biotechnology Progress · Temmuz 2021
YÖKSİS Kayıtları
Preparation of regenerable magnetic nanoparticles for cellulase immobilization: Improvement of enzymatic activity and stability
Biotechnology Progress · 2021 SCI-Expanded
PROFESÖR ELİF ÖZYILMAZ →
Preparation of regenerable magnetic nanoparticles for cellulase immobilization: Improvement of enzymatic activity and stability
Biotechnology Progress · 2021 SCI-Expanded
PROFESÖR MUSTAFA YILMAZ →
Makale Bilgileri
DergiBiotechnology Progress
Yayın TarihiTemmuz 2021
Cilt / Sayfa37
Scopus ID2-s2.0-85102824904
Özet
To obtain regenerable magnetic nanoparticles, triethoxy(3-isocyanatopropyl)silane and iminodiacetic acid (IZ) were used as the starting material and immobilized on Fe3O4 nanoparticles. Copper ions (Cu2+ ions) were loaded on the Fe-IZ nanoparticles and used for cellulase immobilization. The support was characterized by spectroscopic methods (FTIR, NMR) and thermogravimetric analysis, transmission electron microscopy, scanning electron microscope, X-ray diffraction, energy dispersive X-ray analysis, and vibrating sample magnetometer techniques. As a result of experiments, the amount of protein bound to immobilized cellulase (Fe-IZ-Cu-E) and cellulase activity was found to be 33.1 mg/g and 154 U/g at pH 5, 50°C, for 3 h. The results indicated that the free cellulase had kept only 50% of its activity after 2 h, while the Fe-IZ-Cu-E was observed to be around 77%, at 60°C. It was found that the immobilized cellulase maintained 93% of its initial catalytic activity after its sixth use. Furthermore, the Fe-IZ-Cu-E retained about 75% of its initial activity after 28 days of storage. To reuse the support material (Fe-IZ-Cu), it was regenerated by thorough washing with ammonia or imidazole.
Yazarlar (4)
1
Elif Ozyilmaz
2
Ahmet Alhiali
3
Ozge Caglar
4
Mustafa Yilmaz
Anahtar Kelimeler
biocatalysis
cellulase
copper
immobilization
magnetic nanoparticles
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
10
Atıf
4
Yazar
5
Anahtar Kelime