Scopus
YÖKSİS Eşleşti
Rapid mechanochemical production of biocomposites by encapsulating enzymes to zinc based-two metal organic frameworks (ZIF-8 and Zn-MOF-74) for enantioselective hydrolysis reaction of racemic Naproxen methyl ester
Process Biochemistry · Kasım 2023
YÖKSİS Kayıtları
Rapid mechanochemical production of biocomposites by encapsulating enzymes to zinc based-two metal organic frameworks (ZIF-8 and Zn-MOF-74) for enantioselective hydrolysis reaction of racemic Naproxen methyl ester
Process Biochemistry · 2023 SCI-Expanded
PROFESÖR ELİF ÖZYILMAZ →
Makale Bilgileri
DergiProcess Biochemistry
Yayın TarihiKasım 2023
Cilt / Sayfa134 · 276-285
Scopus ID2-s2.0-85174343827
Özet
The mechanochemical encapsulation of enzymes during the synthesis of metal–organic frameworks (MOF) have been thought to be a natural, economical, new, and environmentally friendly step in synthesizing supported enzymatic catalysts. In this study, for the first time, a mechanochemical technique was employed to immobilize Candida rugosa lipase (CRL), and two zinc-based MOF types (Zn-MOF-74 and ZIF-8) were preferred. The enzyme kinetics and immobilization parameters (pH, temperature, thermal stability and reusability) of the prepared encapsulated enzymes (Zn-MOF-74@CRL and ZIF-8@CRL) were investigated. The prepared encapsulated enzymes were characterized by FTIR, SEM and XRD. It was observed that the catalytic activity of Zn-MOF-74 @CRL was six times higher than that of ZIF-8@CRL. After five reuses, the activities of Zn-MOF-74@CRL and ZIF-8@CRL were found to be 81% and 73% retained, respectively. After being subjected to 60 °C for 120 min, ZIF-8@CRL and Zn-MOF-74@CRL maintained their activities at 65% and 73%, respectively. The enantioselective hydrolysis reaction of racemic naproxen methyl ester was chosen as the model reaction. The results showed Zn-MOF-74@CRL possessed better enantioselectivity (E = 245) than ZIF-8@CRL (E = 136). This study demonstrated that it is possible to create biocatalysts with excellent catalytic properties for the pharmaceutical industry using MOFs produced by the mechanochemical method.
Yazarlar (2)
1
Elif Ozyilmaz
2
Ozge Caglar
Anahtar Kelimeler
Biocatalysis
Candida rugosa lipase
Mechanochemical method
Metal organic framework
Naproxen
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
17
Atıf
2
Yazar
5
Anahtar Kelime