Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
Biochemical Engineering Journal · Nisan 2024
YÖKSİS Kayıtları
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
Biochemical Engineering Journal · 2024 SCI-Expanded
Prof. Dr. ELİF ÖZYILMAZ →
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
Biochemical Engineering Journal · 2024 SCI-Expanded
Prof. Dr. MUSTAFA YILMAZ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Universal cell capture by immobilized antimicrobial peptide plantaricin
2015 ISSN: 1369703X SSCI
Prof. Dr. SEMAHAT KÜÇÜKKOLBAŞI →
Universal cell capture by immobilized antimicrobial peptide plantaricin
2015 ISSN: 1369703X SCI-Expanded 1 atıf
Prof. Dr. İLKAY HİLAL GÜBBÜK →
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
2024 ISSN: 1369-703X SCI-Expanded Q2
Prof. Dr. MUSTAFA YILMAZ →
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
2024 ISSN: 1369-703X SCI-Expanded Q2
Prof. Dr. ELİF ÖZYILMAZ →
A novel conductometric creatinine biosensor based on solid state contact ammonium sensitive PVC NH2 membrane
2012 ISSN: 1369703X SCI-Expanded
Dr. Öğr. Üyesi VİLDAN ERCİ →
Makale Bilgileri
ISSN1369703X
Yayın TarihiNisan 2024
Cilt / Sayfa204
Scopus ID2-s2.0-85184885956
Özet
We synthesized the amine-functionalized Zr-MOF (UiO-66-NH2) to provide a covalent binding site for L-proline in the presence of EDC and NHS. Obtained proline-substituted Zr-MOF (UiO-66-NH/Pro) was used as a new carrier for the immobilization of Candida rugosa lipase. To compare catalytic activity, enantioselectivity and stability of the lipase-immobilized UiO-66-NH/Pro (UiO-66-NH/Pro/CRL), lipase was also immobilized on the surfaces of UiO-66-NH2 in the presence of L-proline by physical adsorption to afford UiO-66-NH2/Pro/CRL(enc.). The FTIR, TGA, SEM, EDX, ζ potential and PXRD were performed to assess the structures of the lipase-immobilized Zr-MOFs. Moreover, UiO-66-NH/Pro/CRL and UiO-66-NH2/Pro/CRL(enc.) were successfully used in the hydrolysis reaction of p-nitro-phenylpalmitate (p-NPP). The influence of pH and temperature was also examined. In the optimized conditions, UiO-66-NH/Pro/CRL exhibited higher catalytic activity and enantioselectivity than UiO-66-NH2/Pro/CRL(enc.) in the hydrolysis (UiO-66-NH/Pro/CRL: 64 U/g of activity and 254 of enantioselectivity (E); UiO-66-NH2/Pro/CRL(enc.): 53 U/g of activity and 244 of enantioselectivity (E) reaction. Lineweaver-Burk kinetic studies demonstrated that Vmax value for UiO-66-NH/Pro/CRL was obtained to be higher, whereas, it showed lower Km than UiO-66-NH2/Pro/CRL(enc.). Furthermore, the residual catalytic affinities of UiO-66-NH/Pro/CRL and UiO-66-NH2/Pro/CRL(enc.) in the model hydrolysis reaction were found to be 80% and 60% after five cycles of reuse.
Yazarlar (5)
1
Sebahat Ascioglu
2
Elif Ozyilmaz
3
Ayse Yildirim
4
Serkan Sayin
5
Mustafa Yilmaz
Anahtar Kelimeler
enantioselectivity
kinetic resolution
lipase
metal organic frameworks
UiO-66-NH 2
Kurumlar
Giresun Üniversitesi
Giresun Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Biochemical Engineering Journal
Q2
SJR Skoru0,754
H-Index155
YayıncıElsevier B.V.
ÜlkeNetherlands
Bioengineering (Q2)
Biomedical Engineering (Q2)
Biotechnology (Q2)
Environmental Engineering (Q2)
Metrikler
23
Atıf
5
Yazar
5
Anahtar Kelime