Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
In vitro shoot regeneration of a centaurea amaena boiss. & balansa – A critically endangered and endemic plant
Applied Ecology and Environmental Research · Ocak 2020
YÖKSİS Kayıtları
In vitro shoot regeneration in Centaurea amaena Boiss. & Balansa – critically endangered and endemic plant
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH · 2020 SCI-Expanded
DOÇENT BAYRAM ATASAGUN →
Makale Bilgileri
DergiApplied Ecology and Environmental Research
Yayın TarihiOcak 2020
Cilt / Sayfa18 · 2285-2293
Scopus ID2-s2.0-85084047725
Erişim🔓 Açık Erişim
Özet
This study was conducted to investigate in vitro regeneration potential of Centaurea amaena, a critically endangered and an endemic plant in Turkey. For this purpose, cotyledon, leaf and cotyledon node explants were cultured in Murashige and Skoog (MS) media supplemented with different concentrations of 6-benzylaminopurine (BAP; 1-4 mg L-1), thidiazuron (TDZ; 0.3-1.2 mg L-1) or meta-Topolin (mT; 0.5-4 mg L-1) with or without 0.5 mg L-1 α-naphthalene acetic acid (NAA). In axillary shoot regeneration experiments from cotyledon node explants, the greatest number of shoots per explant (9.975) was obtained from 4 mg L-1 mT-containing MS media, which yielded a shoot regeneration frequency of 70.83%. In indirect organogenesis experiments, the greatest number of shoots per explant in cotyledons (4.152 shoots/explant with the shoot regeneration frequency of 55.00%) was obtained from 1 mg L-1 mT-containing media and the greatest number of shoots per explant in leaves (4.132 shoots/explant with the shoot regeneration frequency of 50.00%) was obtained from 4 mg L-1 mT-containing media. Only callus induction was observed in TDZ-containing media or combinations of TDZ concentrations with NAA. About 50.00% root formation was achieved from half-strength MS medium containing 2.0 mg L-1 indole-3-butyric acid.
Yazarlar (4)
1
S. Uzun
2
E. Ekinci
3
H. Özkatan
4
Bayram Atasagun
Anahtar Kelimeler
Axillary shoot regeneration
BAP
MT
Organogenesis
TDZ
Kurumlar
Erciyes Üniversitesi
Kayseri Turkey
Kayseri University
Kayseri Turkey
Metrikler
1
Atıf
4
Yazar
5
Anahtar Kelime