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Pharmacokinetics, bioavailability and plasma protein binding of tolfenamic acid in rainbow trout (Oncorhynchus mykiss)

Veterinary Medicine and Science · Temmuz 2024

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YÖKSİS Kayıtları
Pharmacokinetics, bioavailability and plasma protein binding of tolfenamic acid in rainbow trout (Oncorhynchus mykiss)
Veterinary Medicine and Science · 2024 SCI-Expanded
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Makale Bilgileri

DergiVeterinary Medicine and Science
Yayın TarihiTemmuz 2024
Cilt / Sayfa10
Erişim🔓 Açık Erişim
Özet Background: Although research on the mechanism and control of pain and inflammation in fish has increased in recent years, the use of analgesic drugs is limited due to the lack of pharmacological information about analgesic drugs. Tolfenamic acid is a non-steroidal anti-inflammatory drug and can be used in fish due to its low side effect profile and superior pharmacokinetic properties. Objectives: The pharmacokinetics, bioavailability and plasma protein binding of tolfenamic acid were investigated following single intravascular (IV), intramuscular (IM) and oral administration of 2 mg/kg in rainbow trout at 13 ± 0.5°C. Methods: The experiment was carried out on a total of 234 rainbow trout (Oncorhynchus mykiss). Tolfenamic acid was administered to fish via IV, IM and oral route at a dose of 2 mg/kg. Blood samples were taken at 13 different sampling times until the 72 h after drug administration. The plasma concentrations of tolfenamic acid were quantified using high pressure liquid chromatography–ultraviolet (UV) and pharmacokinetic parameters were assessed using non-compartmental analysis. Results: The elimination half-life (t1/2ʎz) of tolfenamic acid for IV, IM and oral routes was 3.47, 6.75 and 9.19 h, respectively. For the IV route, the volume of distribution at a steady state and total body clearance of tolfenamic acid were 0.09 L/kg and 0.03 L/h/kg, respectively. The peak plasma concentration and bioavailability for IM and oral administration were 8.82 and 1.24 µg/mL, and 78.45% and 21.48%, respectively. The mean plasma protein binding ratio of tolfenamic acid in rainbow trout was 99.48% and was not concentration dependent. Conclusions: While IM route, which exhibits both the high plasma concentration and bioavailability, can be used in rainbow trout, oral route is not recommended due to low plasma concentration and bioavailability. However, there is a need to demonstrate the pharmacodynamic activity of tolfenamic acid in rainbow trout.

Yazarlar (6)

1
Orhan Corum
ORCID: 0000-0003-3168-2510
2
D. Durna Corum
3
Pedro Marin
ORCID: 0000-0003-4849-4452
4
Omer Faruk Acar
5
Mert Aksoy
6
Kamil Üney
ORCID: 0000-0002-8674-4873

Anahtar Kelimeler

bioavailability pharmacokinetics plasma protein binding rainbow trout tolfenamic acid

Kurumlar

Kastamonu University
Kastamonu Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Murcia
Murcia Spain
University of Hatay Mustafa Kemal
Hatay Turkey

Metrikler

1
Atıf
6
Yazar
5
Anahtar Kelime

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