Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Effect of propolis on pyruvate kinase and superoxide dismutase activities in doxorubicin–induced tissue damage: Molecular docking analysis
Revista Cientifica de la Facultad de Veterinaria · Ocak 2024
YÖKSİS Kayıtları
Effect of propolis on pyruvate kinase and superoxide dismutase activities in doxorubicin–induced tissue damage: Molecular docking analysis
Revista Científica de la Facultad de Ciencias Veterinarias · 2024 SCI-Expanded
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Makale Bilgileri
DergiRevista Cientifica de la Facultad de Veterinaria
Yayın TarihiOcak 2024
Cilt / Sayfa34
Scopus ID2-s2.0-85192997713
Erişim🔓 Açık Erişim
Özet
This study aimed to investigate the effect of propolis on pyruvate kinase (PK) which is a key enzyme in glycolysis and superoxide dismutase (SOD), an antioxidant enzyme on toxicity induced by DOX in different tissues. Using molecular docking, It was looked into how propolis affected the enzymes responsible for glycolysis and the antioxidant system. There was no application in the first group (control). The second group received 100 mg·kg-1 day of propolis by gavage needle for 7 days, a single dose of 20 mg·kg-1 intraperitoneal DOX to the third group, and propolis+DOX to the fourth group. Two days prior to DOX administration, propolis application began, and it lasted for seven days. PK and SOD activities were determined in liver, heart, kidney, and testis tissues, and molecular docking was applied to ratify the activity of some propolis components (caffeic acid phenethyl ester (CAPE) and Quercetin) on PK and SOD enzymes. When the DOX group was compared with the control group, a decrease in PK and SOD activities were found, and significant difference was found in PK and SOD activities. Administration of DOX decreased PK and SOD activities of liver, heart, kidney, and testis tissues. In conclusion, our study reveals that DOX disrupts glycolysis in rat tissues. CAPE and Quercetin compounds were shown to interact similarly with the cocrystal ligands of PK and SOD. In addition, when the interaction types of these compounds especially on PK and the docking scores obtained were examined, it can be said that they show higher affinity than DOX.
Yazarlar (4)
1
Seval Yilmaz
ORCID: 0000-0002-2040-9247
2
Emre Kaya
ORCID: 0000-0002-7445-3091
3
Harun Yonar
4
Harun Uslu
ORCID: 0000-0001-8827-8557
Anahtar Kelimeler
Doxorubicin
molecular docking
pyruvate kinase
superoxide dismutase
toxicity
Kurumlar
Firat Üniversitesi
Elazig Turkey
Selçuk Üniversitesi
Selçuklu Turkey