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THE ANTI HRSV ACTIVITY OF Ferula halophila Peşmen AQUEOUS AND METHANOL EXTRACT BY MTT ASSAY

Trakya University Journal of Natural Sciences · Nisan 2021

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YÖKSİS Kayıtları
THE ANTI HRSV ACTIVITY OF Ferula halophila Peşmen AQUEOUS AND METHANOL EXTRACT BY MTT ASSAY
Trakya University Journal of Natural Sciences · 2021 Emerging Sorches Sciences index
Prof. Dr. HASAN HÜSEYİN DOĞAN →
YÖKSİS ISSN Eşleşmesi

Bu dergide (ISSN eşleşmesi) kurumun 7 kaydı bulundu.

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NOTES ON Arabis kaynakiae Daşkın (Brassicaceae), A CRITICALLY ENDANGERED SPECIES ENDEMIC TO TURKEY
2020 ISSN: 2147-0294 TR DİZİN
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THE MYCOBIOTA OF SAMANLI MOUNTAINS IN TURKEY
2021 ISSN: 2147-0294 Emerging Sorches Sciences index
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THE ANTI HRSV ACTIVITY OF Ferula halophila Peşmen AQUEOUS AND METHANOL EXTRACT BY MTT ASSAY
2021 ISSN: 2147-0294 Emerging Sorches Sciences index
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A NEW TRUFFLE SPECIES ADDITION, TUBER MACROSPORUM, TO TURKISH MYCOTA
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Biological activity evaluation of three Hypericum species wild growing in Sivas province of Turkey
2022 ISSN: 2147-0294 Web of Science - Emerging Sources Citation Index (ESCI)
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Natural Treatment Approaches For Varicose Veins: A Brief Review Of The Literature
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Makale Bilgileri

ISSN21470294
Yayın TarihiNisan 2021
Cilt / Sayfa22 · 43-48
Erişim🔓 Açık Erişim
Özet The trend towards natural products in the world is increasing due to the increased drug resistance of infectious diseases, the high prices for drugs and the difficulty of access. Also, bacterial or viral diseases that are difficult to treat and need a long time for proper treatment cause important infections in people. Since effective drugs could not be developed for most viral infections, it is very important to find natural products against viruses to introduce them to the world of science. The antiviral activities of the aqueous and methanol extract from Ferula halophila Peşmen, an endemic species to Turkey, has been investigated against human respiratory syncytial virus (HRSV, ATCC-VR-26). The cytotoxic and antiviral properties of plant extracts were investigated in HRSV / HEp-2 cell systems, respectively by the colorimetric MTT assay. In the study, the titer of RSV was used as 100 TCID50 (50% tissue culture infective dose). While MNTC (Maximum non-toxic concentration) of methanol extract was 195.313 µg/mL and CC50 (50% cytotoxic concentration) was 4366.22 µg/mL, it was determined as MNTC = 390.625 µg/mL and CC50 = 4366.22 for aqueous extract. According to the results, methanol extract showed more toxicity than the aqueous extract. The MNTC of Ribavirin used as a positive control was determined as 0.98 µg/mL and CC50 was 110.40 µg/mL. As a result of the study, it was determined that the methanol extract was not effective, whereas the aqueous extract had a significant anti-HRSV activity with the values of 50.69 µg/mL EC50 (50% Effective Concentration) and 97.54 Selectivity Index (SI). The EC50 and SI values of Ribavirin were 2.39 µg/mL and 46.19, respectively. According to the results, we can explain the presence of antiviral activity only in the aqueous extract and the absence in the methanol extract by the high toxicity of it and the insolubility of the antiviral compounds in the methanol extract.

Yazarlar (2)

1
Hasan Huseyin Dogan
2
Rüstem Duman

Anahtar Kelimeler

Antiviral activity Cytotoxicity Ferula halophila Human respiratory syncytial virus Methanol and aqueous extract

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Trakya University Journal of Natural Sciences
Q4 OA
SJR Skoru0,225
H-Index7
ÜlkeTurkey
Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q4)
Molecular Biology (Q4)
Dergi sayfasına git

Metrikler

8
Atıf
2
Yazar
5
Anahtar Kelime