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A simple isocratic high-perfomance liquid chromatography method for the simultaneous determination of shikonin derivatives in some Echium species growing wild in Turkey

Turkish Journal of Pharmaceutical Sciences · Nisan 2018

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YÖKSİS Kayıtları
A Simple Isocratic HPLC Method for Simultaneous Determination of Shikonin Derivatives in Some Echium Species Wild Growing in Turkey
The Turkish Journal of Pharmaceutical Sciences · 2018 ESCI
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Makale Bilgileri

DergiTurkish Journal of Pharmaceutical Sciences
Yayın TarihiNisan 2018
Cilt / Sayfa15 · 38-43
Erişim🔓 Açık Erişim
Özet Objectives: Dried roots of Echium species are used in Turkey for the treatment of wounds, inflammation, and depression. In this study, an reversed-phase-liquid chromatographic method with isocratic elution was developed to determine shikonin derivatives in Echium species. Materials and Methods: The chromatographic separation and quantification was performed on a C18 column (ACE, 150 mm × 4.6 mm, 5 μm), with a mobile phase of acetonitrile and 0.1 M acetic acid (70: 30, v/v) at a flow rate of 1 mL/min, and ultraviolet detection at 520 nm. Results: Linear behavior was observed over the investigated concentration range (2-500 ppm) for all analytes, with a correlation coefficient of >0.998. The proposed method was found to be specific and precise for the quantification of shikonin derivatives in Echium species. Conclusion: The highest content of shikonin derivatives was observed in E. italicum L. compared with the other species studied herein, advocating the use of E. italicum L. roots as an alternate source for shikonin derivatives.

Yazarlar (1)

1
Nuraniye Eruygur

Anahtar Kelimeler

Echium sp HPLC Shikonin derivatives

Kurumlar

Cumhuriyet Üniversitesi
Sivas Turkey
Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

10
Atıf
1
Yazar
3
Anahtar Kelime

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