Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Determination of 1-Deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of In-vitro antioxidant, α-Amylase and α-Glucosidase inhibitory activity in mulberry varieties from Turkey
Phytomedicine · Şubat 2019
YÖKSİS Kayıtları
Determination of 1-Deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of In-vitro antioxidant, α-Amylase and α-Glucosidase inhibitory activity in mulberry varieties from Turkey
Phytomedicine · 2019 SCI-Expanded
Prof. Dr. NURANİYE ERUYGUR →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Protective effects of curcumin supplementation on intestinal ischemia reperfusion injury
2013 ISSN: 09447113 SSCI
Prof. Dr. MUAZ BELVİRANLI →
Chemical and Biological Insights on Cotoneaster integerrimus A new epicatechin source for food and medicinal applications
2016 ISSN: 09447113 SCI-Expanded
Prof. Dr. AHMET UYSAL →
Chemical and biological insights on Cotoneaster integerrimus A new epicatechin source for food and medicinal applications
2016 ISSN: 09447113 SCI
Prof. Dr. ABDURRAHMAN AKTÜMSEK →
Protective effects of curcumin supplementation on intestinal ischemiareperfusion injury
2013 ISSN: 0944-7113 SSCI 7 atıf
Prof. Dr. NİLSEL OKUDAN →
Chemical and biological insights on Cotoneaster integerrimus A new epicatechin source for food and medicinal applications
2016 ISSN: 09447113 SCI-Expanded
Dr. Öğr. Üyesi ERDOĞAN GÜNEŞ →
In vitro and in silico perspectives on biological and phytochemical profile of three halophyte species—A source of innovative phytopharmaceuticals from nature
2018 ISSN: 0944-7113 SCI-Expanded
Prof. Dr. GÖKHAN ZENGİN →
Determination of 1-Deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of In-vitro antioxidant, α-Amylase and α-Glucosidase inhibitory activity in mulberry varieties from Turkey
2019 ISSN: 0944-7113 SCI-Expanded
Prof. Dr. NURANİYE ERUYGUR →
Mechanisms of action of cytotoxic phenolic compounds from Glycyrrhiza iconica roots
2019 ISSN: 0944-7113 SCI-Expanded
Prof. Dr. YÜKSEL KAN →
Mechanisms of action of cytotoxic phenolic compounds from Glycyrrhiza iconica roots
2019 ISSN: 0944-7113 SCI
Prof. Dr. YÜKSEL KAN →
Makale Bilgileri
Dergi
Phytomedicine
ISSN09447113
Yayın TarihiŞubat 2019
Cilt / Sayfa53 · 234-242
Scopus ID2-s2.0-85055827459
Özet
Background: Morus alba and Morus nigra leaves which have been widely used as herbal teas in Anatolian region of Turkey, were extracted twice by 50 mM HCI solution, derivatized with 9-fluorenylmethyl chloroformate and analyzed by reversed phase HPLC equipped with a fluorescence detector. Hypothesis/Purpose: This study was performed to determine the main antidiabetic active compounds 1-deoxynojirimycin by HPLC method and evaluate the in-vitro antioxidant and antidiabetic activity of ethanol extracts prepared from Morus alba L. and Morus nigra leaves. Study Design: A reliable simple, and rapid high-performance liquid chromatographic (HPLC) method for the determination of 1-deoxynojirimycin in M. alba L. and M. nigra leaves with fluorimetric detection after pre-column derivatization with 9-fluorenylmethyl chloroformate was developed. In addition, the chemical composition of ethanol extract of mulberry leaves was analyzed with GC–MS. Methods: Separation and quantitation were performed on C18, 250 × 4.6 mm, 5 µm analytical column. Mobile phase consisted of acetonitrile and 0.1% acetic acid solution (1:1, v/v) was performed applied to the column 1.0 ml/min flow rate at 26 °C. Potential antioxidant activity of ethanol extract of different mulberry varieties were evaluated by DPPH, and ABTS radical scavenging assay as well as total phenol and flavonoid content were determined. In addition, α-amylase and α-glucosidase activity was determined by 96-well plate method to evaluate the probable antidiabetic potential use of Turkish mulberry leaves. Results: The isocratic HPLC method showed excellent correlation coefficient (r 2 = 0.9985) between 0.3 and 30 µg/ml calibration points. The method was specific and sensitive with detection and quantification limits of 1.07 and 3.27 ng/ml, respectively. Intraday and interday method precision (n = 5) were < 7.3 (RSD%). Intraday and interday method accuracy (n = 5) were between 3.77 and (−8.35) (RE%). The average method recovery (n = 3) was 102.5%. The results showed that the content of 1-deoxynojirimycin in leaves of Morus alba L. was 0.103% (n = 3), and in leaves of M. nigra L. was 0.102%. 2-hexadecen-1-ol, oleamide, 2-propenoic acid, and cyclododecane were identified as the major compounds by GC–MS in the ethanol extract of mulberry leaves. Conclusion: The obtained robustness values from emission and excitation detection, mobile phase ingredients and flow rates changes showed that method was very strong. This work contributes to the knowledge of antioxidant and antidiabetic properties of Morus species, thus may be provide useful data in evaluation of food products and pharmaceutical preparations produced from Morus species.
Yazarlar (2)
1
Nuraniye Eruygur
2
Emrah Dural
Anahtar Kelimeler
1-deoxynojirimycin
Antidiabetic activity
Antioxidant
FMOC-Cl derivatization
GC–MS
HPLC- fluorescent detection
Morus sp.
Kurumlar
Cumhuriyet Üniversitesi
Sivas Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Phytomedicine
Q1
SJR Skoru2,092
H-Index160
YayıncıElsevier GmbH
ÜlkeGermany
Complementary and Alternative Medicine (Q1)
Drug Discovery (Q1)
Molecular Medicine (Q1)
Pharmaceutical Science (Q1)
Pharmacology (Q1)
Metrikler
49
Atıf
2
Yazar
7
Anahtar Kelime