Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen

Makale Bilgileri

DergiCryobiology

Yayın TarihiHaziran 2023

Cilt / Sayfa111 · 104-112

DOI10.1016/j.cryobiol.2023.04.003

Özet Azeri water buffalo is a species of great interest due to the high quality of its products such as milk. Due to the decreasing trend of its number and risk of extinction in the future, our attention is directed towards ensuring the preservation of its genetic reserves by keeping its sperm. Using antioxidants in semen extender is one of the ways to reduce the detrimental effects of freezing process on post-thawed quality of spermatozoa. This study was conducted to determine the effect of κ-carrageenan (k-CRG) and C60HyFn supplemented semen extender on the quality of post-thawed Azari water buffalo spermatozoa. A total of 30 semen samples were obtained from three buffaloes using an artificial vagina (twice a week for five weeks = 10 replicates). The samples (n = 3) from each replicate were pooled and divided into equal aliquots to prepare 14 extender groups, including control (C), k-0.2, K-0.4, K-0.6, K-0.8 (containing 0.2, 0.4, 0.8 mg K-CRG/mL, respectively), C-0.1, C-0.2, C-0.4, C-0.8, C-1, C-5, C-10, C-20, and C-40 (containing 0.1, 0.2, 0.4, 0.6, 0.8, 1, 5, 10, 20, 40 μM C60HyFn, respectively), and then frozen. After thawing, motility and velocity parameters, plasma membrane integrity (PMI) and functionality (PMF), DNA damage, Hypo-osmotic swelling (HOS) test, malondialdehyde (MDA), total antioxidant capacity (TAC), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase glutathione activities and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging were evaluated. In vivo fertility was compared between k-0.6, C-1 and control groups. 60 buffalo were inseminated 24 h after the onset of estrus. The diagnosis of pregnancy was performed rectally at least 60 days after fertilization. Total and progressive motility and velocity parameters were improved by k-0.4, k-0.6, k-0.8, C-0.4, C-0.8, C-1, C-5, and C-10 groups) compared to the other groups. Plasma membranes integrity and PMF were improved by k-0.4, k-0.6, C-0.4, C-0.8, C-1, C-5, and C-10 groups compared to other groups, while in terms of sperm DNA damage K-0.4, K-0.6, K-0.8, C-0.2, C-0.4, C-0.8, C-1, C-5, and C-10 groups showed better results compared to the control group. The evidence also showed that k- 0.4, k-0.6, k-0.8, C-0.4, C-0.8, C-1, C-5, and C-10 groups could improve TAC, and decrease MDA levels. Also, k-0.4, k-0.6, k-0.8, C-0.2, C-0.4, C-0.8, C-1, C-5, and C-10 groups could improve GPx, CAT, and GSH levels, but no significant difference was found regarding SOD compared to the other groups. DPPH scavengers were tested by K-0.6, K-0.8 and C-1, C-5, C-10, C-0.8, C-0.4 and C-0.2 groups and compared to other groups improved. The fertility rate [70% (14/20)] was higher in C-1 than other groups. To conclude that k-CRG and C60HyFn supplementation can increase the quality parameters of cryopreserved buffalo semen after thawing and that 1 M C60HyFn can increase in vivo fertility of buffalo semen.

Yazarlar (10)

1

Negin Ramazani

2

Farid Mahd Gharebagh

3

Ali Soleimanzadeh

ORCID: 0000-0002-1591-2198

4

Esin Keles

5

Desislava Georgieva Gradinarska-Yanakieva

ORCID: 0000-0003-1096-0972

6

Damla Arslan-Acaröz

7

Mahdi Zhandi

ORCID: 0000-0003-2886-9747

8

A. Baran

9

Esmail Ayen

10

D. A. Dinc

ORCID: 0000-0002-9597-227X

Anahtar Kelimeler

Buffalo C60HyFn Cryopreservation Oxidative stress Semen quality κ-carrageenan

Kurumlar

ACR Bio

Afyonkarahisar Turkey

Afyon Kocatepe Üniversitesi

Afyonkarahisar Turkey

Istanbul University-Cerrahpasa

Istanbul Turkey

Kyrgyz-Turkish Manas University

Bishkek Kyrgyzstan

Researcher in Biology and Animal Reproduction

Urmia Iran

Selçuk Üniversitesi

Selçuklu Turkey

T.C. Tarim ve Köyişleri Bakanliği

Antakya Turkey

The Bulgarian Academy of Sciences

Sofia Bulgaria

University of Tehran

Tehran Iran

Urmia University Faculty of Veterinary Medicine