Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
In vitro investigation of cytotoxic and apoptotic effects of Cynara L. species in colorectal cancer cells
Asian Pacific Journal of Cancer Prevention · Aralık 2013
YÖKSİS Kayıtları
In vitro Investigation of Cytotoxic and Apoptotic Effects of Cynara L Species in Colorectal Cancer Cells
Asian Pacific Journal of Cancer Prevention · 2013 SCI-Expanded
DOÇENT ELA NUR ŞİMŞEK SEZER →
In vitro Investigation of Cytotoxic and Apoptotic Effects of Cynara L Species in Colorectal Cancer Cells
ASIAN PACIFIC JOURNAL of CANCER PREVENTION · 2013 SCI-Expanded
PROFESÖR TUNA UYSAL →
Makale Bilgileri
DergiAsian Pacific Journal of Cancer Prevention
Yayın TarihiAralık 2013
Cilt / Sayfa14 · 6791-6795
Scopus ID2-s2.0-84892458617
Erişim🔓 Açık Erişim
Özet
Apoptotic and cytotoxic activity of plant extracts obtaining from naturally growing Cynara syriaca in Turkey and cultivated C cardunculus against DLD1 colorectal cancer cells was determined. Extracts from wild and cultivated Cynara species were obtained from their vegetative parts and receptacles using hexane and applied with five different dose (0.1-1 mg/ml) as well as apigenin for MTT tests for three time periods (24, 48 and 72 hours). After cells were treated with IC50 doses for each extract total DNA and RNA were isolated for determination of the cause of cell death. From isolated RNAs, cDNA were synthesized and amplification of p21, BCL-2 and BAX gene regions was carried out. Consequently, we found that pro-apoptotic (BAX) gene expression and a cell cycle inhibitor (p21) were induced in the presence of our artichoke extracts. In contrast, anti-apoptotic BCL-2 gene expression was reduced compared to the control group. In addition DNA fragmentation results demonstrated DLD1 cell death via apoptosis.
Yazarlar (2)
1
Ela Nur Simsek
2
Tuna Uysal
Anahtar Kelimeler
Anti-cancer agent
Artichoke
CDNA
Colorectal cancer
DLD1
DNA fragmentation
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
27
Atıf
2
Yazar
6
Anahtar Kelime