Scopus
YÖKSİS Eşleşti
Pulsed-field gel electrophoresis typing, antibiotic resistance, and plasmid profiles of Escherichia coli strains isolated from foods
Canadian Journal of Microbiology · Eylül 2012
YÖKSİS Kayıtları
Pulsed field gel electrophoresis typing antibiotic resistance and plasmid profiles of Escherichia coli strains isolated from foods
Canadian Journal of Microbiology · 2012 SCI-Expanded 1 atıf
PROFESÖR AHMET UYSAL →
Makale Bilgileri
DergiCanadian Journal of Microbiology
Yayın TarihiEylül 2012
Cilt / Sayfa58 · 1278-1287
Scopus ID2-s2.0-84869049769
Özet
Bacterial contamination in foods and antimicrobial resistance levels of common pathogenic strains causing foodborne disease are important in human health. Thus, typing technologies are important tools to determine primary sources of bacterial contamination. In this study, 40 Escherichia coli strains isolated from 85 food samples were evaluated in terms of genetic diversity, susceptibility to certain antibiotics, and plasmid profiles. Pulsed-field gel electrophoresis was used to identify the genetic relations of E. coli isolates. It was determined that the 40 E. coli strains revealed 32 different pulsotypes represented by 6 subtypes. Antibiotic susceptibility tests conducted by using a disc diffusion method against 15 antibiotics showed that although the isolates revealed 14 different types of resistance profiles, the strains showed the greatest resistance to ampicillin (77.5%), followed by ticarcillin- clavulanic acid (30%), tetracycline (22.5%), and cephalothin (14.5%). Plasmid isolations studies of the strains conducted by the method of alkaline lysis revealed that 18 (45%) of 40 E. coli strains contain 31 different plasmid bands ranging between 64.4 and 1 kb. The results showed that PFGE was a powerful method in tracking sources of food contamination and that the antibiotic resistance levels of food isolates were high and should be monitored.
Yazarlar (2)
1
Ahmet Uysal
2
Yusuf Durak
Anahtar Kelimeler
Antibiotic resistance
Escherichia coli
Genetic diversity
Plasmid profiles
Pulsed-field gel electrophoresis
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
2
Yazar
5
Anahtar Kelime