Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Bruguiera gymnorhiza (L.) lam. at the forefront of pharma to confront zika virus and microbial infections—an in vitro and in silico perspective
Molecules · Ekim 2021
YÖKSİS Kayıtları
Bruguiera gymnorhiza (L.) Lam. at the Forefront of Pharma to Confront Zika Virus and Microbial Infections\u2014An In Vitro and In Silico Perspective
Molecules · 2021 SCI-Expanded
PROFESÖR AHMET UYSAL →
Makale Bilgileri
DergiMolecules
Yayın TarihiEkim 2021
Cilt / Sayfa26
Scopus ID2-s2.0-85115725148
Erişim🔓 Açık Erişim
Özet
The recent emergence of Zika virus (ZIKV) in Brazil and the increasing resistance developed by pathogenic bacteria to nearly all existing antibiotics should be taken as a wakeup call for the international authority as this represents a risk for global public health. The lack of antiviral drugs and effective antibiotics on the market triggers the need to search for safe therapeutics from medicinal plants to fight viral and microbial infections. In the present study, we investigated whether a mangrove plant, Bruguiera gymnorhiza (L.) Lam. (B. gymnorhiza) collected in Mauritius, possesses antimicrobial and antibiotic potentiating abilities and exerts anti-ZIKV activity at non-cytotoxic doses. Microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 70603, methicillin-resistant Staphylococcus aureus ATCC 43300 (MRSA), Salmonella enteritidis ATCC 13076, Sarcina lutea ATCC 9341, Proteus mirabilis ATCC 25933, Bacillus cereus ATCC 11778 and Candida albicans ATCC 26555 were used to evaluate the antimicrobial properties. Ciprofloxacin, chloramphenicol and streptomycin antibiotics were used for assessing antibiotic potentiating activity. ZIKVMC-MR766NIID (ZIKVGFP) was used for assessing anti-ZIKV activity. In silico docking (Autodock 4) and ADME (SwissADME) analyses were performed on collected data. Antimicrobial results revealed that Bruguiera twig ethyl acetate (BTE) was the most potent extract inhibiting the growth of all nine microbes tested, with minimum inhibitory concentrations ranging from 0.19–0.39 mg/mL. BTE showed partial synergy effects against MRSA and Pseudomonas aeruginosa when applied in combination with streptomycin and ciprofloxacin, respectively. By using a recombinant ZIKV-expressing reporter GFP protein, we identified both Bruguiera root aqueous and Bruguiera fruit aqueous extracts as potent inhibitors of ZIKV infection in human epithelial A549 cells. The mechanisms by which such extracts prevented ZIKV infection are linked to the inability of the virus to bind to the host cell surface. In silico docking showed that ZIKV E protein, which is involved in cell receptor binding, could be a target for cryptochlorogenic acid, a chemical compound identified in B. gymnorhiza. From ADME results, cryptochlorogenic acid is predicted to be not orally bioavailable because it is too polar. Scientific data collected in this present work can open a new avenue for the development of potential inhibitors from B. gymnorhiza to fight ZIKV and microbial infections in the future.
Yazarlar (11)
1
Nabeelah Bibi Sadeer
ORCID: 0000-0002-0602-0059
2
Juliano G. Haddad
3
Mohammed Oday Ezzat
4
Philippe Desprès
5
Hassan H. Abdallah
6
Gokhan Zengin
ORCID: 0000-0001-6548-7823
7
Ahmet Uysal
8
Chaker El Kalamouni
9
Monica Gallo
10
Domenico Montesano
ORCID: 0000-0002-8857-2091
11
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
ADME
Antimicrobial
Antiviral
Envelope protein
Mangrove plants
Pharmacokinetics
Kurumlar
Salahaddin University-Erbil
Erbil Iraq
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Napoli Federico II
Naples Italy
Université de La Réunion
Saint-Denis France
University of Anbar
Ramadi Iraq
University of Mauritius
Reduit Mauritius
Metrikler
6
Atıf
11
Yazar
6
Anahtar Kelime