Scopus
YÖKSİS Eşleşti
Protective Effect of Nigella sativa and Nigella damascena Fixed Oils Against Aflatoxin Induced Mutagenicity in the Classical and Modified Ames Test
Chemistry and Biodiversity · Ekim 2021
YÖKSİS Kayıtları
Protective Effect of Nigella sativa and Nigella damascena Fixed Oils Against Aflatoxin Induced Mutagenicity in the Classical and Modified Ames Test
CHEMISTRY & BIODIVERSITY · 2021 SCI
PROFESÖR YÜKSEL KAN →
Protective Effect of Nigella sativa and Nigella damascena Fixed Oils Against Aflatoxin Induced Mutagenicity in the Classical and Modified Ames Test
Chemistry & Biodiversity · 2021 SCI-Expanded
PROFESÖR YÜKSEL KAN →
Protective Effect of Nigella sativa and Nigella damascena Fixed Oils Against Aflatoxin Induced Mutagenicity in the Classical and Modified Ames Test
Chemistry & Biodiversity · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ İREM AYRAN ÇOLAK →
Makale Bilgileri
DergiChemistry and Biodiversity
Yayın TarihiEkim 2021
Cilt / Sayfa18
Scopus ID2-s2.0-85113290749
Özet
The antioxidant and mutagenic/antimutagenic activities of the fixed oils from Nigella sativa (NSO) and Nigella damascena (NDO) seeds, obtained by cold press-extraction from the cultivar samples, were comparatively investigated for the first time. The antimutagenicity test was carried out using classical and modified Ames tests. The fatty acid composition of the fixed oils was characterized by gas chromatography–mass spectrometry (GC-MS) while the quantification of thymoquinone in the fixed oils was determined by UPC2. The main components of the NSO and NDO were found to be linoleic acid, oleic acid, and palmitic acid. The results of the Ames test confirmed the safety of NSO and NDO from the viewpoint of mutagenicity. The results of the three antioxidant test methods were correlated with each other, indicating NDO as having a superior antioxidant activity, when compared to the NSO. Both NSO and NDO exhibited a significant protective effect against the mutagenicity induced by aflatoxin B1 in Salmonella typhimurium TA98 and TA100 strains. When microsomal metabolism was terminated after metabolic activation of the mycotoxin, a significant increase in antimutagenic activity was observed, suggesting that the degradation of aflatoxin B1 epoxides by these oils may be a possible antimutagenic mechanism. It is worthy to note that this is the first study to assess the mutagenicity of NSO and NDO according to the OECD 471 guideline and to investigate antimutagenicity of NDO in comparison to NSO against aflatoxin.
Yazarlar (9)
1
Sinem Helvacıoğlu
2
Mohammad Charehsaz
3
Etil Guzelmeric
ORCID: 0000-0001-9696-3271
4
Mehmet Ali Oçkun
ORCID: 0000-0001-8180-7399
5
İrem Ayran
6
Hasan Kırmızıbekmez
7
Yüksel Kan
8
Ahmet Aydın
9
Erdem Yesilada
Anahtar Kelimeler
antimutagenic activity
antioxidants
mutagenesis
Nigella damascene
Nigella sativa
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Yeditepe University
Istanbul Turkey
Metrikler
9
Atıf
9
Yazar
5
Anahtar Kelime